An Introduction to rhBMP-2

rhBMP-2
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rhBMP-2
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rhBMP-2
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Biologics International Corp (BIC) is a specialist in rhBMP-2 production.
We have delivered more than 200 batches of rhBMP-2 to our world-wide customers.
Also, we guarantee the best price and high quality.
Cat. No.: CY115
Full Name: Bone Morphogenetic Protein-2 Human Recombinant
Synonyms: BMP-2; BMP2; Bone Morphogenetic Protein 2; BMP2A
Source: E. coli
Amino Acid Sequence: The sequence of the N-terminal amino acids was determined as Met-Gln-Ala-Lys-His.
Molecular Weight: Bone Morphogenetic Protein-2 Human Recombinant was expressed in E. coli as a homodimeric, non-glycosylated polypeptide chain containing 115 amino acids. BMP2 exhibits a Molecular Weight of approximately 26 kDa as revealed by SDS-PAGE (under non-reducing conditions).
Purity: >98% as determined by SDS-PAGE and HPLC
Endotoxin: Endotoxin level was found to be <0.1 ng/µg(1EU/µg) by a LAL gel clot method.
Formulation: Lyophilized from a 50mM acetic acid solution, filtered by 0.2 µm membrane.
Reconstitution: To reconstitute the protein, add sterile 100mM AcOH (acetic Acid) to the lyophilized protein and make a preparation with a concentration ≥0.1mg/ml. The preparation can be diluted into other appropriate buffers if necessary. Cautions should be taken to avoid bacterial growth, resulting in potential contamination with endotoxin.
Stability: The lyophilized protein is stable at room temperature (≈3 weeks) and at -20°C (≥ 2 years from the date of receipt).Upon reconstitution, it can be stored at 4°C (≤ 7 days) or at -20°C (≤ 6 months). For long term storage, please store at -80°C and the addition of a carrier protein (0.1% HSA or BSA) is recommended.Avoid frequent freeze-thaw cycles.
Biological Activity: Determined by its ability to induce alkaline phosphatase production in ATDC-5 cells. The ED50 is <50ng/ml.
Usage: FOR LABORATORY RESEARCH USE ONLY. Not intended for diagnostic or therapeutic purposes.
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rhBMP-2 Activity

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rhBMP-2 SDS-PAGE

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rhBMP-2 Mass Spec


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