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Bone Morphogenetic Protein-2 Human Recombinant
BMP-2; BMP2; Bone Morphogenetic Protein 2; BMP2A
Amino Acid Sequence:
The sequence of the N-terminal amino acids was determined as Met-Gln-Ala-Lys-His.
Bone Morphogenetic Protein-2 Human Recombinant was expressed in E. coli as a homodimeric, non-glycosylated polypeptide chain containing 115 amino acids. BMP2 exhibits a Molecular Weight of approximately 26 kDa as revealed by SDS-PAGE (under non-reducing conditions).
>98% as determined by SDS-PAGE and HPLC
Endotoxin level was found to be <0.1 ng/µg(1EU/µg) by a LAL gel clot method.
Lyophilized from a 50mM acetic acid solution, filtered by 0.2 µm membrane.
To reconstitute the protein, add sterile 100mM AcOH (acetic Acid) to the lyophilized protein and make a preparation with a concentration ≥0.1mg/ml. The preparation can be diluted into other appropriate buffers if necessary. Cautions should be taken to avoid bacterial growth, resulting in potential contamination with endotoxin.
The lyophilized protein is stable at room temperature (≈3 weeks) and at -20°C (≥ 2 years from the date of receipt).Upon reconstitution, it can be stored at 4°C (≤ 7 days) or at -20°C (≤ 6 months). For long term storage, please store at -80°C and the addition of a carrier protein (0.1% HSA or BSA) is recommended.Avoid frequent freeze-thaw cycles.
Determined by its ability to induce alkaline phosphatase production in ATDC-5 cells. The ED50 is <50ng/ml.
FOR LABORATORY RESEARCH USE ONLY. Not intended for diagnostic or therapeutic purposes.