Hybridoma Antibody Sequencing

Hybridoma antibody sequencing is generally based on reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) technologies. RACE is an effective method based on PCR, which rapidly amplifies the 5′ and 3′ ends of cDNA from a low number of transcripts, and is widely used because it is simple, fast and inexpensive.
Biologics International Corp. (BIC) has years of experience in hybridoma antibody sequencing. First, we will extract your mRNA from your hybridoma cells and conduct RT-PCR to obtain the cDNA. Then, the light chain, heavy chain, or full-length fragment will be amplified by PCR or 3′ and 5′ RACE. The fragments can be connected to T-vectors and sequenced. The sequencing range covers mice, rats, rabbits, goats, chickens, and other species. BIC has a mature antibody preparation platform, and also provides a recombinant antibody production service.

Service Content

Description Customer Provides Deliverables Timeline Price
Leader Sequence &
Variable Domain Sequencing 
  • Antigen information
    or hybridoma cells
    (>1 × 106 cells)
  • Detailed work performance report
  • Sequence data
  • Plasmid containing antibody gene sequence
  • 3~4 weeks Please inquire
    Full-Length Antibody Sequencing 

    Why to Sequence a Hybridoma Antibody

    • Articles and patents: Antibody sequencing data are necessary to distinguish new antibodies from other antibodies.
    • Antibody recombination: The antibody sequence is indispensable to produce high-quality, highly stable, and low-cost recombinant antibodies simply and rapidly.
    • Antibody humanization: Antibody sequence data are essential for antibody humanization, to rescue the antibody gene before hybridoma cell lines fail to grow or Ab production is lost.

    What We Offer

    • Professional technology: RT-PCR and RACE combined.
    • Rigorous scientific research attitude: More than five positive clones will be sequenced to avoid pseudogenes interference.
    • Various sequencing species: Covering mice, rats, rabbits, goats, chickens, and other species.
    • Comprehensive service content: Providing hybridoma antibody sequencing (genetic level) and antibody sequencing services (amino acid level).

    Workflow

    hybridoma-antibody-sequencing

    Related Services

    Contact Us

    phone+1 (317) 703-0614
    fax +1 (855) 427-1516
     For questions and/or a price quote, please inquire online or fill in a pre-order form.
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