Mammalian Cell Expression System

The rapid progress of molecular cell biology has created a need for structural biologists to look at increasingly more complex protein structures. These usually require particular post-translational modifications and co-factors to support their elaborate folding and enzymatic activity. Prokaryotes are unable to perform these modifications and lack essential eukaryotic co-factors.

Mammalian cell expression exhibits the highest level of protein processing, which is important for complete biological activity. Therefore, the mammalian expression system is suitable for expressing complicated, modified, functional proteins/antibodies; secreted or membrane proteins, such as recombinant glycoproteins (vaccines and enzymes), hormones, antibodies, interleukins and lymphokines.

Owing to the low expression level of mammalian systems and the complex components of the medium, the expenses incurred for obtaining large amounts of protein are relatively high, which in turn poses a challenge in achieving large amounts of protein. With the top technical team and unique optimization scheme in BIC, we can promptly evaluate and identify the optimal expression conditions to meet your diverse needs.

BIC offers a 5% discount for all online inquiries! Contact us today and communicate with our scientists.

Highlights

  • Rapid expression of functional protein or antibody.
  • Customization: quantity, purity, endotoxin, and RNase level are optional along with any other services you may request.
  • Modifications: isotope labeling, protein biotinylation, and green fluorescent labeling services.
  • Our services advance your project from transient to stable expression.
  • Expression scale-up: from 1 to 100 L

Mammalian Expression Service

Content Service Process Deliverables Timeline
Gene synthesis
  • Codon optimization
  • Gene synthesis
  • Subcloning & Max-Prep
  • DNA sequencing data
  • Recombinant expression vector DNA (1ug)
  • Expression & purification results
  • QC data
2-3 weeks
Transient
Transfection
Expression
Pilot Expression:

  • Expression test
  • Purification test
  • Western blot analysis

Scale Up:

  • Protein/Antibody expression (0.2-1L)
  • Purification: affinity chromatography, gel filtration, ion-exchange
  • QC: SDS-PAGE & Western blot,
    mass spectrometry (optional)
4-6 weeks
Stable Transfection Expression Stable Transfection Cell Line Construction

The Process of Protein or Antibody Production in Mammalian Cell

mammalian cell expression system

Additional Services

Based on our customized solutions and extensive experience, we provide the following two optional services:

Protein Labeling

Protein labeling technique refers to the covalent attachment of molecules such as enzymes to specific sites on the target protein. BIC has developed a series of protein labeling techniques, including fluorescent probes (FITC), enzyme conjugates (horseradish peroxidase and alkaline phosphatase), isotope labeling and biotinylation.

Large Scale Production

The WAVE bioreactor is the ideal cell culture equipment. WAVE utilizes a sterile and closed disposable bag, that can effectively prevent contamination. In addition, WAVE can automatically control process conditions such as incubation temperature, pH, dissolved oxygen, and pressure.

Case Study

  • Recombinant protein production in mammalian cell
  • mammalian-system-case-15kda

    Figure 1. 15kDa protein purification

    Lane M: Protein marker; Lane 1: Culture supernatants of transfected mammalian cells; Lane 2: Protein purification, flow-through; Lane 3-5: Elution with different imidazole concentration buffers

    Figure 2. Glycosylation modified protein expression

    Lane M: Protein marker; Lane 1: Culture supernatants of transfected mammalian cells; Lane 2: Protein purification, flow-through; Lane 3-5: Elution with different imidazole concentration buffers

  • Recombinant antibody production in mammalian cell
  • Figure 3. Human IgG expression and purification

    Lane M: Protein marker; Lane 1: Culture supernatants of transfected mammalian cells; Lane 2: Antibody purification, flow-through; Lane 3-5: Glycine elution

    Figure 4. Fab antibody expression and purification

    Lane M: Protein marker; Lane 1: Culture supernatants of transfected mammalian cells; Lane 2: Fab antibody purification, Flow-through; Lane 3: Glycine elution (Reduced); Lane 4: Glycine elution (Non-reduced)

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