Bacterial Protein Expression Service

Biologics International Corp (BIC) provides custom protein expression services. We have extensive experience in bacterial, mammalian cell, and yeast expression systems and provide highly purified active proteins in scales from large to small tailored to your needs. For more information on our bacterial expression service, please see our Guaranteed, SupernatantIN™, and Tag-free protein production packages.

Guaranteed
Service Package

Guaranteed bacterial Service

  • 4 weeks, one stop service from sequence to protein.
  • Risk-Free: Get your desired quantity and purity or your money back.
  • We prioritize purify protein from supernatant.

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SupernateIN™
Service Package

supernateIN bacterial expression service

  • 9 host bacteria
  • 6 molecular chaperones
  • 9 soluble tags
  • dozens of vectors
  • Flexible temperatures and induction strategies.
  • We deliver supernatant protein.

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Tag-free
Service Package

Tag Free service

  • Minimum or no effects on target protein function or interactions.
  • Close to native protein crystal structure.
  • Identical sequences and similar structures with native proteins.

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Bacterial Fermentation Service

Standard fermentation: 5-50 mg protein/L (or 7-17 g wet cell): starting ONLY from $390.
High cell density fermentation: 20-400 mg protein/L (or 20-120 g wet cell): starting ONLY from $690.

Endotoxin Removal Service

Endotoxin Level Options <1.0 EU/µg (Guaranteed), start from $300, 1~2 weeks.

High-throughput Protein Expression Service

Our high-throughput capacity enables us to provide bulk protein with high quality in short turnaround time.

  • One-step protein purification by Magnetic Agarose Beads.
  • High throughput expression in 96/24 deep-well formats.
  • 10-100 μg purified recombinant proteins.

Bacterial Protein Expression Technology Platforms

BIC offers highly sophisticated bacterial protein expression technologies to provide products tailored to your needs.

codon optimization

Codon and mRNA structure optimization

Our in house informatics tools enable us to enhance expression of any given recombinant protein by adjusting codon usage and frequency of its gene; and by opening up stem-loops of the gene during transcription and translation.Read More >

protein Aliquot and lyophilisation platform

Vector construction

Our professional teams can help you construct a customized vector tailored to your research requirement (often affected by upstream sequences and downstream applications).

protein refolding platform

Multiple purification methods

Nickel affinity purification is our preferred method. Ion exchange purification, size-exclusion chromatography, and hydrophobic chromatography are also available in our protein purification service. We can also provide favorable protocols to optimize your project process. Read More >

protein identification platform

Multi-channel protein refolding

We possess state-of-the-art protein refolding technologies, includingtraditional dialysis and dilution methods, as well as on-column refolding generally used in large-scale protein production.Using multi-well platestoexplore refolding conditions, our teams can find the most effective conditions for obtaining stably and uniformly refolded proteins with good solubility. Read More >

protein purification platform

MALDI-TOF

We provide MALDI-TOF MS, one of the most useful attributes in protein identification, to accurately determine the molecular weight of your protein of interest.

protein sequencing platform

Protein N-terminal sequencing

Our highly sensitive N-terminal sequencing technology enables us to quickly determine cleavage sites and confirm the identity of purified protein samples.

Aliquot and lyophilisation

To increase the stability of expressed recombinant proteins and reduce shipping cost, we provide aliquoting and lyophilisation service for long-term storage of your products.

Contact Us

phone+1 (317) 703-0614
fax +1 (855) 427-1516
 For questions and/or a price quote, please inquire online or fill in a pre-order form.
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