Stable Cell Line Services

Biologics International Corp (BIC) provides stable cell line generation services, including stable cell line for research assay, CHO stable cell line for protein production, CRISPR gene knockout cell line service. We can simplify your cell line development process and save your research and development time.

Why Develop Stable Cell Lines

Stable cell lines can grow continuously over a long period of time without significant change in the expression level of the target gene. It is widely used in functional studies of genes, drug screening and production of proteins, for both therapeutic and non-therapeutic purposes.

  • Functional studies: the expression level of a gene in certain cells can be manipulated to study its functions.
  • Drug screening: stable cell lines are very important for drug screening in a combinatorial chemical library.
  • Proteins for research: proteins for research purposes can be produced by transient transfection of HEK293 cells. However, this method is not very cost effective in producing recombinant proteins in large quantity. For some highly demanded proteins, a stable cell line is more desirable as production host.
  • Proteins/antibodies for pharmaceutical: stable cell lines are essential for FDA approval and useful for preparing recombinant proteins, recombinant antibodies and vaccines for therapeutic or preventive purposes.

How to Develop Stable Cell Lines

  • Cell preparation
  • Determination of antibiotic screening concentration
  • Plasmid construction and cell transfection
  • Screening, detection and amplification of positive clones
  • Pressure selection
  • Subcloning and single clone screening
  • Quality control of cell lines

Need more information? Please do not hesitate to get in touch.

Contact Us

phone+1 (317) 703-0614
fax +1 (855) 427-1516