GC content is usually calculated as a percentage value and sometimes called G+C ratio or GC-ratio. GC-content percentage is calculated as Count(G + C)/Count(A + T + G + C) * 100%.
The GC content calculation algorithm has been integrated into our Codon Optimization Software, which serves our protein expression services.
Ángela Patricia Guerra, et al. "Producción de proteínas recombinantes de Plasmodium falciparum en Escherichia coli" Revista Biomédica.
The GC pair is bound by three hydrogen bonds, while AT pairs are bound by two hydrogen bonds. And so,
The GC content affects the stability of DNA.
The GC content affects the secondary structure of mRNA.
The GC content affects the annealing temperature for template DNA in PCR experiments.
The GC Content can be used in
1. primer design for PCR experiments
2. gene design from protein expression
3. mRNA hairpin prediction