Custom Antibody Production Service

Biologics International Corp (BIC) offers an extensive line of custom antibody production services, including mouse and rat custom antibodies. Our professionals work to fully understand customers’ requirements (testing, specifications, packaging, regulatory requirements), and then our scientists help researchers to produce custom antibodies per the customer’s specifications in a timely, quality-controlled, and flexible manner.

We specialize in high-quality antibodies, particularly those which are difficult to produce. Our main foci are robust screening methodologies and excellent antigen design. Antigen design and production are key factors in the successful development of a high-quality antibody. We have considerable experience in protein production and peptide synthesis.

Our antibody products are backed by a 100% satisfaction guarantee to work in diverse applications, including western blot (WB), flow cytometry (FC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), as antibody-drug conjugates, and as ELISA detection antibodies (direct, indirect, sandwich, and capture ELISA formats).

Our Custom Antibody Services:

BIC provides monoclonal antibody production services, protein/peptide antigen design, and antibody labeling, among others. If the delivered antibody fails to meet the standard requirements, there will be no charge to the customer. Our internal antigen design tools ensure a high antibody production success rate and the largest natural epitope of soluble antigen. We guarantee an ELISA titer >1:128,000, a purity >90%, and positive test results in WB.

Custom Monoclonal Antibody

Biologicscorp's polyclonal antibody service is designed to supply you with custom, affinity-purified pAbs that are guaranteed to detect your antigen of choice using ELISA (titer >1:128,000) and dot blot assays. If no pAb is delivered, there is no charge.

Custom Polyclonal Antibody

W provide an antibody pairing service. Paired antibodies can bind to the same antigen at the same time and are commonly used in double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). The number of immunized mice is at least 10 (basic service), and the pairing success rate is high. BIC offers complete upstream and downstream support services including antigen synthesis, preparation of monoclonal antibody, antibody pairing, and mass production of antibodies. You only need to provide antigen or antigen sequences, and BIC will deliver the paired antibodies.

Antibody Pairing

Other Antibody Service

BiologicsCorp offers additional custom antibody services, including antibody labeling, antibody purification, antibody subtype identification, and antibody affinity determination. These specialized antibody services can be further tailored to meet your custom projects and needs.

How to Select Antibodies

Two factors should be taken into consideration before the start of each project design. One is the intended application of the monoclonal, polyclonal, and single-domain antibodies. The other is the choice of host species. The following table provides advantages and disadvantages of each antibody type related to these two factors.

Projects Selection

Projects Pros Cons Species
custom-antibody-monoclonal-antibody-production

Monoclonal Antibody
  • Unlimited quantity and availability of antibody
  • Lot-to-lot consistency
  • High specificity
  • Quick reproduction of antibody once hybridoma is developed
  • Longer production time
  • Requires a minimum of 4 mg immunogen
  • Higher cost of development
  • Antibodies less likely to work for multiple applications
  • More difficult if using peptide immunogens
  • Low affinity
  • Mouse
  • Rabbit
custom-antibody-polyclonal-antibody-production-1

Polyclonal Antibody
  • Quick turnaround
  • Inexpensive
  • High affinity
  • One antibody can work for a variety of applications
  • Requires less than 1mg immunogen
  • Multiple host species, including non-mammalian hosts
  • Better at detecting denatured proteins
  • High requirement for the purity of immunogen
  • Requires a minimum of 4 mg immunogen
  • Prone to produce non-specific antibodies, and produce background signals in some applications. Quantity available limited by the number of bleeds
  • Lot-to-lot variability in characterization
  • Difficulty to reproduce
  • Chicken
  • Goat
  • Rabbit
  • Guinea Pig
  • Mouse
  • Rat
  • Sheep

Species Selection

Species Advantages Disadvantages
Mouse
  • Common host species for antibody generation
  • Small amount of antigen required
  • Easy initial assays
  • Low cost
  • Not suitable for short peptide antigens
  • Small amount of serum
Rabbit
  • Good host species for antibody production
  • High response to peptide and most protein antigens
  • Broad epitope recognition
  • Moderate cost
  • Not ideal for highly conserved mammalian proteins
Rat
  • Small amount of antigen required
  • Ideal host for mouse antigen
  • Easy initial assays
  • Good for small-volume polyclonal antibody projects
  • Low cost
  • Small amount of serum
Goat
  • Good for large volume polyclonal antibodies
    Large volume antibody production
  • Large mammalian host species
  • Easy initial assays
  • Not suitable for modified peptides
  • Large antigen quantities
  • High cost
Chicken
  • High specificity against antigen
  • Suitable for polyclonal antibody production
  • Good yield
  • Moderate cost
  • Difficult to isolate antibodies
  • Not ideal for the most peptide antigens

FAQs About Custom Antibody Production

Below are some frequently asked questions about our antibody production services. Should you have any questions other than those listed below, feel free to contact us, and we will be happy to answer them.

Antibody Information


  1. What does the customer need to submit to BIC before the start of the project?
  2. Below are some frequently asked questions about our antibody production services. Should you have any questions other than those listed below, feel free to contact us, and we will be happy to answer them.

  3. What types of antibody service does BIC offer?
  4. The standard antibody production service at BIC includes rabbit polyclonal antibody production and monoclonal antibody production. Meanwhile, other services can also be provided, such as antibody pairing, antibody labeling, antibody purification, antibody subtype identification, and antibody affinity determination.

  5. Does BIC guarantee antibody titers?
  6. Yes, our guaranteed antibody service begins with antigen design and ends with antibody purification. We guarantee the delivery of antibody titers >1:128,000 and positive WB test results. There will be no charge to the customer if these requirements are not met.

  7. What species do you provide for your antibody production service?
  8. For polyclonal antibodies, we provide two New Zealand white rabbits and for monoclonal antibodies, 5 BALB/c mice. If there is a need for other species, such as chickens, sheep, guinea pigs, rats, etc., or if immunization of additional animals is required, please contact us.

  9. What antibody quality testing reports are delivered?
  10. Progress Reports.(Includes ELISA and Western Blot data against peptide/protein).

Antigen Information


  1. What are your antigen requirements?
  2. If you are submitting your own antigen for immunization, the requirements vary depending on the project type. For protein antigen, 3–5 mg protein is needed (with a purity of 90%, and a concentration of 0.5 mg/ml).

  3. Do you offer antigen design and production?
  4. Yes, we design and produce protein/peptide antigens and deliver samples (100 μg). Moreover, we provide these services at a lower cost than our leading competitors. Please consult us for more details.

  5. Do you use a carrier (hapten) when immunizing with a peptide antigen?
  6. Yes, we will conjugate the peptide to KLH (keyhole limpet hemocyanin) as a carrier protein for injection with our peptide antigens.

Others


  1. How will the antibody be shipped?
  2. The final purified antibody/antigen will be shipped as a lyophilized powder.

  3. How long is the project expected to take?
  4. Prior to initiation of the custom antibody project, BIC will provide the customer with an estimate of project milestone dates on the sales order. However, the schedule as specified on the sales order is an estimate, and the actual time may vary depending on the project.

  5. Is the project confidential?
  6. BIC and the customer agree that no confidential information, including but not limited to project scope, contract terms, pricing information, product development technologies and processes, and business-related information, shall be disclosed to any third party, and both parties receiving confidential information shall only make internal use of said confidential information.

  7. Do you offer volume discounts?
  8. Yes, please inquire with our sales representatives at sales@biologicscorp.com.

Need more information? Please do not hesitate to get in touch.

Contact Us

phone+1 (317) 703-0614
fax +1 (855) 427-1516
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