Recombinant Antibody Expression Service

Biologics International Corp (BIC) has extensive expertise and research experience in the field of recombinant antibody expression.Till date, we have successfully delivered more than 2,000 recombinant proteins/antibodies (until July 1st, 2017). We can produce recombinant antibodies in a wide range of formats including IgG/D/E, scFv, Fab/Fabʹ, sdAb/VHH, Fc fusion proteins, chimeric antibody, antibody sequencing and even more, which are sure to meet your requirements. Simply provide us with a gene sequence, and we’ll take care of the rest. With our top technology and optimization solutions, we guarantee the quantity and the quality of the antibody, and offer a no-success no-fee guarantee.

BIC offers a 5% discount for all online inquiry! For further information please contact us, we will be happy to assist you.

Recombinant Antibody Expression

Human IgG

Mouse IgG
Human IgD
Human IgE

To mimic the behavior of a recombinant human IgG antibody (targeting a human protein) in humans the best, specificity-matching recombinant mouse IgG antibodies derived from phage display mouse scFv/Fab libraries (targeting the mouse protein) are frequently produced and used in animal models. We use a modified vector to produce recombinant antibodies in HEK293 or CHO cells for transient or stable expression. Recombinant antibody service will offer antibodies with >90% purity and a QC report. The service covers a wide range of research needs from synthesis of a gene to highly purified antibodies within a duration of as less as 7 weeks. For further information or pricing of the recombinant antibody service, please contact us.

Recombinant Antibody Fragment Expression

recombinant-antibody expression

scFv

Compared with mAbs, scFv’s have a small size and offer several advantages, including low kidney uptake, rapid blood clearance, greater tumor penetration, and less negative response from the human immune system. scFv fragments could be expressed in both bacterial cells and mammalian cells. Our service provides scFv construction via two different methods, i.e., scFv produced from synthetic DNA or via accession number.

Fab/Fabʹ

Fab fragments differ from scFv’s as they are composed of one constant and one variable domain of each of the heavy and the light chains. Fab fragments can be created using papain, which could cleave an immunoglobulin monomer into two Fab fragments and an Fc fragment. If the variable domains had been sequenced, the fragments could be produced by recombinant expression.

sdAb/VHH

A single-domain antibody (sdAb, VHH, or nanobody) is a type of antibody fragments consisting of a single monomeric variable antibody domain. With a molecular weight of only 12–15 kDa, single-domain antibodies are much smaller than common antibodies (150–160 kDa), and even smaller than scFv fragments (25 kDa): offer some advantages, including outstanding penetrability and high stability, and can be expressed in both eukaryotic and prokaryotic systems.

Fc Fusion Proteins

Fc fusion proteins are composed of an immunoglobulin Fc domain that is directly linked to another peptide or protein of interest. Fc fusion proteins can play a role in dimerization, in vivo half-life, and receptor activation and affinity for the cognate ligand.

And More

We also have the ability to generate a range of antibody fragments and other formats. Contact us to discuss your exact requirements.

Service Content

Custom Provides Recombinant Antibody Expression Deliverables Timeline
Antibody sequences

Gene synthesis

Vector construction

Transient expression & purification

Cell line production (optional)

4 ug gene in cloning vector

>90% purity antibody

Quantity as per customer
requirements (>0.1 mg)

QC report

7-9 weeks
Need more information? Please do not hesitate to get in touch.

Contact Us

phone+1 (317) 703-0614
fax +1 (855) 427-1516
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