Recombinant Antibody Expression

Biologics International Corp (BIC) has extensive expertise and research experience in the field of recombinant antibody production. Till date, we have successfully delivered more than 2,200 recombinant antibodies/proteins (until July 1st, 2017). We can produce recombinant antibodies in a wide range of formats including full-length antibodies, scFv, Fab/Fabʹ, sdAb/VHH, Fc fusion proteins, and chimeric antibody. Simply provide us with a gene sequence or hybridoma cells, and we’ll take care of the rest with the top technology and optimization solutions.

BIC offers a 5% discount to academic institutions on all custom services.

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Service Highlights

  • Guaranteed: no-success no-fee guarantee.
  • Customizable: quantity, purity, tags, and labels are customizable. We can also produce a wide rang of recombinant antibody formats.
  • One-stop service: our one-stop service covers from hybridoma sequencing to antibody expression.

Service Content

Customers provide Procedure Deliverables Timeline
Two options:

  • Antibody sequence
  • Hybridoma cells
Hybridoma sequencing (optional)
  • Sequencing report
3 weeks
  • Gene synthesis
  • Subcloning
  • Transient transfection
  • 4 μg gene in cloning vector
2-3 weeks
  • Expression and purification
  • Western blot and ELISA
  • 1-3 mg antibody with >90% purity
  • QC report
3-4 weeks

Notes:

BIC can offer following services or options to meet the specific requirements of our esteem customers:

  1. Stable cell line construction: cell lines are the best option for long-term antibody production.
  2. Large scale antibody production: we can deliver 0.1-1 g recombinant antibodies if you required.
  3. Antibody labeling: several labeling molecules are available, such as biotin, fluorescein isothiocyanate (FITC), horseradish peroxidase (HRP).
  4. Antibody engineering: we have ability to construct scFv or Fab antibodies using phage display technology.

For further information please, contact us, we will be happy to assist you.

Formats of Recombinant Antibody

Recombinant antibodies have a wide rage of formats, such as full-length antibody, scFv, Fab/Fab’, sdAb, Fc fusion protein, etc. Each of the antibodies has different potential in applications and research.

Recombinant IgG antibodies are frequently produced and used in animal models, aiming to mimic the behavior of a recombinant human IgG antibody (targeting a human protein) in humans the best. scFv has many advantages as its small size, including low kidney uptake, rapid blood clearance, greater tumor penetration, and less negative response from the human immune system. Fab/Fab' fragments differ from scFv as they have constant domains. sdAb has a molecular weight of only 12-15 kDa which offers several advantages, including outstanding penetrability and high stability. Fc fusion proteins can play a role in dimerization, in vivo half-life, and receptor activation and affinity for the cognate ligand.

BIC has the ability to generate these antibodies with high quality and efficiency.

Need more information? Please do not hesitate to get in touch.

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phone+1 (317) 703-0614
fax +1 (855) 427-1516
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