Recombinant Antibody Production Service

Biologics International Corp (BIC) provides recombinant antibody production services. BIC has extensive expertise and research experience in the field of recombinant antibody production. We have established a solid platform offering the expression of a range of recombinant antibodies, including IgG, IgD, IgE, nanobody (VHH), scFv, di-scFv, Fab, F(ab'), chimeric antibody, Fc fusion protein and more. We are so confident in our antibody production services, we offer a no-success no-fee guarantee.

Expression System

We have CHO and HEK293 cells for recombinant antibody production in the mammalian system.

Key Features

  • Guaranteed or your money back: we have launched a guaranteed recombinant antibody expression service.
  • Competitive prices: as low as $2,500, including the cost of gene synthesis, with no additional charge.
  • High success rate: codon optimization and high expression vector, success rate >95%.

What We Offer

The following full-length antibodies and fragments can be produced by BIC:

Antibody Fragment Introduction
scFv/di-scFv
scFv/di-scFv
  • scFv consisting of the VH and VL domains linked by a flexible peptide
  • Di-scFv is produced by connecting two scFv molecules through a short linker
  • It retains the complete activity and stability of the whole Ig molecule with higher assay sensitivity
Nanobody(VHH)
Nanobody(VHH)
  • Nanobody is the single variable domain of the antibodies heavy-chain
  • It has high affinity and specificity, whereas the immunogenicity is very low
  • It is the smallest naturally found antigen-binding region
 Fab/F(ab’)
Fab/F(ab’)
  • Fab is composed of one constant domain and one variable domain of each of the heavy and light chains (VH and CL)
  • It can stably combine with the antigen
Full-length Antibody Introduction
IgG/IgD/IgE
Ig
  • IgG antibody has four isotypes
  • You need to choose the light chain - κ & λ
  • We provide production of recombinant IgG, IgE and IgD
Chimeric
Chimeric
  • Chimeric antibody fuses variable domains from your antibody
  • It can be combined with any constant domain from a different species
  • It retains the original affinity and specificity of the parent
Fc fusion protein
Fc fusion protein
  • It is composed of the Fc domain of IgG linked to the protein of your interest
  • Fusion an active protein with Fc can extend in vivo half-life with better protein stability

Two options for recombinant antibody expression:

recombinant antibody production

Recombinant Antibody Production Service

Recombinant antibody production service description

Customer provides Recombinant antibody production service Deliverables Timeline
Recombinant antibody sequences Gene synthesis Codon optimization
  • 4 ug gene in cloning vector
  • >90% purity antibody
  • Quantity as per customer requirements
  • QC report
7-9 weeks
Vector construction
Misc. Sub-cloning &
DNA max-prep
Antibody production Recombinant antibody expression
Antibody purification Protein A/G purification
QC SDS-PAGE & Western blot

Self-developed high-level expression system

Codon optimization was run to remove potential negatively acting sequences and to modify codon usage in recombinant antibody production. High-yield expression achieved is attributable to sequence optimization, which leads to improved efficiency of mRNA translation.

  • Strong promoter

To meet increasing demand on high quality recombinant antibody, we developed a novel vector with optimized promoter activity, which contributed to higher expression level compared with traditional CMV based vector.

  • Dual-expression vector

Our increasing expression level of secreted recombinant antibody production is based on the self-developed mammalian dual expression vector. It comprises two different genes encoding two polypeptide chains (e.g. the heavy and light chains of target recombinant antibody). The method solves the problem of the plasmid with imbalanced heavy and light separately and leads to a considerable enhanced expression level. Host cell line that best fits the system is also available to improve recombinant antibody yield.

Key features of recombinant antibody production

  • High-specificity: bind to corresponding antigen specifically with high-affinity
  • Non-animal technology: alleviate animal welfare concerns
  • Fast speed: significantly shorter than conventional immune antibody production
  • Easy-to-manipulate: capable of guiding recombinant antibody production process
  • Isotype conversion: can be easily converted into any antibody isotype (e.g. IgA, and IgG etc.) from any species by adding the appropriate constant domain
  • Broad target antibody: antibodies to highly toxic or non-immunogenic antigens can be created
  • Amenable to high-throughput production: attractive to the field of proteomics

Real-time QC: antigen-antibody reaction analysis

The Octet® system (Octet® RED96) based on Bio-Layer Interferometry (BLI) was developed as a promising biosensor platform to detect binding specificity of interacting molecules, including recombinant antibody and corresponding antigens. Processed curves, KD value and other reaction rate constants will be provided after real-time, label-free measurements.

Real-time QC: antigen-antibody reaction analysis

Contact Us

phone+1 (317) 703-0614
fax +1 (855) 427-1516
 For questions and/or a price quote, please inquire online or fill in a pre-order form.
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