The positive correlation between degree of codon bias and level of gene expression has been proved. This rare codon analysis tool is just to plot the codon usage frequency of your sequence and shows the codon usage distribution. It can help you decide if your sequence needs to be optimized for heterologous gene expression.
this tool has been integrated into our Codon Optimization Software, which serves our protein expression services.
Agarwal, Shivangi, et al. "Induced autoprocessing of the cytopathic Makes caterpillars floppy-like effector domain of the Vibrio vulnificus MARTX toxin." Cellular microbiology (2015).