Co-Immunoprecipitation (CO-IP)

Co-Immunoprecipitation (CO-IP) is an effective method to identify intracellular protein-protein interaction by using the bait protein antibody to indirectly capture target proteins that bind to the bait protein.
Biologics International Corp (abbr. BIC) provides CO-IP service. Our technical group will take the protein’s concentration and expression level into consideration when designing the optimal proposal. Meanwhile BIC also has a mature recombinant expression platform. We can provide protein expression service when the target protein is identified.

Why CO-IP?

  • The interaction of proteins is in a natural state.
  • The process of protein interaction is carried out in the natural state which can avoid the impact of man-made.
  • The interacted protein complex can be obtained in the natural state.

CO-IP Process

CO IP

Service Content

Service Name Deliverables Timeline Price
Bait protein expression cloning vector, QC report, protein with the purity>90% ~4 weeks Please inquire
CO-IP Interaction analysis report, protocol report ~2 weeks Please inquire
Target protein preparation (optional) cloning vector, QC report, 3-5 mg purified protein (purity>90%) 4~6 weeks Please inquire

What You Need to Provide:

Information Antibody
Options:

  • Gene sequence of bait protein: You provide the sequence of bait protein, and BIC prepare protein. If your target protein is known, you also need to provide the sequence of target protein.
  • Plasmid of bait protein: You provide plasmid with bait protein and sequencing diagram files.If your target protein is known, you also need to provide the plasmid of target protein.
  • At least 5 mL lysate: Containing both bait protein and target protein.
Options:

  • Customer provides the detective antibody>20 μL.
  • BIC prepare relevant antibody.

Case Study

co-ip-s

SDS-PAGE FigureSilver Staining Figure

  • Lane 1: anti-X antibody
  • Lane 2: protein X-anti-X antibody
  • Lane 3: protein X-anti-X antibody-agarose beads protein A binded effluence
  • Lane 4: protein X-anti-X antibody-agarose beads protein A elution
  • Lane 5: protein X-agarose beads protein A binded effluence
  • Lane 6: protein X-agarose beads protein A elution
  • Lane 7-12: SDS-PAGE corresponding silver staining figure

Related Service

  • If your protein is tagged with GST, you may choose our GST Pull Down Service.
  • If you want to prepare protein, you may select our Guaranteed Protein Expression Service.
  • Contact Us

    phone+1 (317) 703-0614
    fax +1 (855) 427-1516
     For questions and/or a price quote, please inquire online or fill in a pre-order form.
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