Bacterial Protein Prodction Troubleshooting

Bacterial Protein Prodction Troubleshooting No or Low Protein Production This situation may be regarded as the worst case scenario. When the protein of interest cannot be detected through a sensitive technique(e.g.,Western blot) or it is detected but at very low levels(less than micrograms per liter of culture), the problem often lies in a harmful effect […]

Bacterial Endotoxin Definition

Bacterial Endotoxin Definition What are endotoxins Bacterial Endotoxin Definition:┬áLipopolysaccharides (LPS), also known as lipoglycans and endotoxins, Endotoxins are part of the outer membrane of the cell wall of Gram-negative bacteria. Although the term “endotoxin” is occasionally used to refer to any cell-associated bacterial toxin, in bacteriology it is properly reserved to refer to the lipopolysaccharide […]

Bacterial Expression System

Bacterial Expression System During the proteomics period, the growth in the use of recombinant proteins has increased greatly in the recent years. Bacterial expression systems remain most attractive due to low cost, high productivity, and rapid use. Escherichia coli expression system continues to dominate the bacterial expression systems and remain to be the preferred system […]