Overview of Protein Refolding

Overview of Protein Refolding Recombinant protein expression technology is widely used in protein functional research. Heterologous gene expression commonly leads to production of inclusion bodies. To obtain souble protein, inclusion bodies must be refolded into an active form through refolding methods. Refolding is initiated by reducing the concentration of denaturant to solubilize inclusion bodies. What […]

Chemical Protein Synthesis Definition

Chemical Protein Synthesis Definition Chemical protein synthesis is named in contrast to the biological protein synthesis. The synthesis is by coupling the carboxyl group or C-terminus of one amino acid to the amino group or N-terminus of another. It is also called peptide synthesis, because the length of synthesized amino acid sequence is limited by current […]

Protein Synthesis vs Protein Production

Protein Synthesis vs Protein Production Protein synthesis is the nature biological process with two big steps, transcription and translation. The transcription is from DNA to mRNA and the translation is from mRNA to protein. The protein production is not a totally nature biological process, which integrates recombinant DNA technology and the nature biological protein synthesis […]

Transient Transfection Introduction

Transient Transfection Introduction Transient transfection is a well-established method to rapidly express recombinant proteins from mammalian expression system. Transient transfections can be reliably and readily scaled up to handle milliliters to tens of liters of cells in suspension culture and obtain milligrams to grams of recombinant protein in a process that requires only days to […]

High Cell Density Fermentation – A Efficient Fed-Batch Fermentation Technology

High Cell Density Fermentation – A Efficient Fed-Batch Fermentation Technology High Cell Density Fermentation VS Stand Large Scale Fermentation High cell density fermentation by fed-batch strategies is one of the most cost-effective means of achieving high yields for the production of large scale recombinant proteins in the bio-industry. In fed-batch cultures, cell mass and productivity […]

Soluble Protein Test Introduction and Method

Soluble Protein Test Introduction and Method Introduction of soluble protein test: Pure, soluble and functional proteins are of high demand in modern biotechnology. Natural protein sources rarely meet the requirements for quantity, ease of isolation or price and hence recombinant technology is often the method of choice. Recombinant cell factories are constantly employed for the production of protein […]

Recombinant Protein Articles

Recombinant Protein Articles   Production of Recombinant Proteins – Challenges and Solutions (PDF) Abstract: Efficient strategies for the production of recombinant proteins are gaining increasing importance,as more applications that require high amounts of high-quality proteins reach the market. Higher production efficiencies and, consequently, lower costs of the final product are needed for obtaining a commercially […]

History of Recombinant Protein

History of Recombinant Protein What is the History of Recombinant protein? The method of recombinant DNA was initially planned by a graduate student, Peter Lobban, along with a biochemist, A. Dale Kaiser at the Stanford University. During then years,1972–74, the method was then acknowledged by Stanley Norman Cohen, an American geneticist Chang, Herbert Boyer, a […]

Recombinant Protein Definition

Recombinant Protein Definition Customize Recombinant Proteins In-stock Recombinant Proteins Analyze your Proteins Recombinant protein is a manipulated form of protein, which is generated in various ways to produce large quantities of proteins, modify gene sequences and manufacture useful commercial products. The formation of recombinant protein is carried out in specialized vehicles known as vectors. Recombinant […]