High-throughput Protein Production Service

The emerging field of functional proteomics is driving the need to develop high-throughput technology to express and isolate a large number of proteins quickly for comprehensive analyses. Biologics International Corp (BIC) offers an innovative high-throughput protein production for bacterial protein expression system (typically several hundreds to thousands are involved), therefore to free up your time and speed up your projects.

Highlights of High-throughput Protein Production Service

  • High throughput capability towards gene synthesis and expression
  • One-step protein purification by Magnetic Agarose Beads
  • Fast and cost-effective methods for delivering multiple purified proteins

High-throughput Protein Production Service Description

Project process Descriptions Timeline Deliverables
High-throughput Protein Production Service Process Multiple gene synthesis & subcloning in parallel (optional)
  • Codon optimization
  • HTP gene synthesis
  • Subcloning into E.coli expression vectors
<2 weeks
  • Sequencing chromatograms covering your genes (electronic)
  • expression constructs
  • 10-100 μg purified recombinant proteins (in 96-deep well plates)
  • QC data of proteins (SDS-PAGE or dot blot)
High-throughput protein expression
  • High-throughput protein expression in 96- or 24-deep-well block formats
  • Detect expression level by SDS-PAGE or dot blot
<1 week
High-throughput protein purification
  • Automated purification process on Magnetic Agarose Beads
<1 week

Additional services: Bacterial Expression System, Tag-free Protein, Fermentation Service, Endotoxin Removal

High-throughput Technology

  • Affinity tags enable simultaneous purification for highly dissimilar proteins with the use of one standardized procedure, which is essential for high-throughput technologies.
  • The development of plate-based purification strategies that allow the rapid capture of 24-96 different proteins and their subsequent purification by affinity resins has greatly expedited multi-parallel applications.
  • One-step and automated purification process on Magnetic Agarose Beads has also been another major contribution for high-throughput protein production. The highly specific and irreversible binding to the beads enables efficient protein capture regardless of the expression level and eliminates protein loss during wash steps, resulting in high protein recovery.
High-throughput technology
Fig 1. Micro-scale protein purification in 96-well format with Ni-NTA Magnetic Agarose Beads

Your Concerns

1. What starting materials do I have to provide?

All we need is the sequence information of your target proteins or genes. We can start from gene synthesis with codon optimized for protein expression, and deliver final purified proteins. If you would like us to start with your plasmids/expression strains, you are welcome.

2. Is there any limit for proteins expression in high-throughput technology?

Successful high-throughput protein production generally requires target proteins between 10kDa to 150kDa, and signal peptide and multiple transmembrane domains should be removed for improved expression yield.

3. I have 35 proteins production, could I choose your high-throughput protein production service?

BIC can accept a batch of 20 to 2000 constructs. Please contact us for more information.

Need more information? Please do not hesitate to get in touch.

Contact Us

phone+1 (317) 703-0614
fax +1 (855) 427-1516