Labeling Service

As an acknowledged biotechnology provider, Biologics International Corp. (BIC) offers a range of custom antibody and protein labeling services, which are performed by highly skilled labeling professional teams. Labeling molecules include biotin, isotope, horseradish peroxidase (HRP), and fluorescein isothiocyanate (FITC). Choosing the best conjugate of antibody or protein for your application may be not easy. Please contact us for a consultation and let us work with you to pick the best label to suit your specific needs.

Service Highlights

• Diverse selection: We offer a wide variety of labels, including enzymatic labels (HRP) and non-enzymatic labels (isotope, FITC, and biotins).
• Full technical support: With years of experience, our professional teams can be your reliable partner to provide the labeled antibodies or proteins with high bioactivity and sensitivity.

Service Contents

Service Item	Deliverables	Timeline	Price
Biotin Labeling	1-20 mg labeled antibodies or proteins	1 week	Inquire
Isotope Labeling
HRP Labeling
FITC Labeling

Have any questions? Send a message now! Let our experts help pick the correct label and simplify the process!

Antibody and Protein Labeling

Antibody labeling is an important technique to aid in the detection or isolation and purification of a protein. Labeled antibodies are essential tools in applications of biological research and diagnostics, such as western blots, enzyme-linked immunosorbent assay (ELISA), immunofluorescence (IF), and immunohistochemistry (IHC).
Protein labeling refers to the use of the appropriate molecular labels to facilitate the detection or purification of a labeled protein and its binding partners.

Reporter enzymes (such as HRP) can be attached to antibodies or proteins using different coupling chemistries to ensure the maximum retention of activity of both the enzyme and the protein. Non-enzymatic biotin, isotopes, and FITC can be coupled to specific antibodies, proteins, and peptides as a means of signal generation.

Biotin

Biotin is a small molecule (244.3 Da) with a wide distribution in animal and plant tissues. As the affinity of avidin or streptavidin for biotin is one the strongest known non-covalent interactions, biotin-labeled antibodies can increase the sensitivity of an assay and be employed in detection (such as ELISA, western blot, IF, and IHC).
At BIC, we are able to offer two different methods of biotin antibody labeling, i.e. AviTagTM technology (enzymatic biotinylation) and chemical biotinylation. Please visit our biotinylation service page to learn more details.

Isotopes

With the development of mass spectrometer and computers, the stable isotope labeling technique provides a vision for the understanding of absolute information about all proteins in a biological sample. There are two popular approaches; one is the absolute quantification method (AQUA) using stable isotope-labeled peptides as internal standards, and the other is metabolic stable isotope labeling in amino acids in cell culture (SILAC).

BIC provides isotope labeling services using our optimized methods. Till data, we have provided more than 100 isotope-labeled proteins with an isotope enrichment c.a. of 98%. For any further information regarding isotope labeling, please contact us, we are always glad to assist you.

HRP

HRP-labeled antibodies can react with specific substrates, generating a chromogenic, chemiluminescent, or a fluorescent signal upon the oxidation ability of HRP, which makes HRP to be one of the most commonly used enzyme labels in the area of antibody-based antigen detection assays.

We can provide rapid, efficient, and high-quality custom HRP labeling services. Consult our HRP labeling service page for detailed information.

FITC

Fluorescent dyes have a wide range of selections, which provides greater flexibility, variation, and performance for research applications. Fluorescein, based on its amine-reactive isothiocyanate derivative FITC, has been one of the most popular fluorophores because of its high absorptivity, excellent fluorescence quantum yield, and good water solubility. Unlike biotin or HRP, FITC molecule could be utilized alone and does not require additional reagents for detection, which makes FITC extremely versatile for monitoring biological processes in vivo.

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