Guaranteed Protein Expression Service in E.coli

Biologics International Corp (BIC) offers risk-free E.coli protein expression services according to your research needs. Using information regarding only your protein sequences, we can deliver your protein of interest in the specified quantity and purity. To ensure smooth and successful completion of your project, a dedicated PhD-level technical support staff will be assigned to your project from initial inquiry to post-delivery. Our scientists will work closely with you and update project progress while taking all your goals into account. Bulk order discounts are available.

Service Highlights

  • Guaranteed service: No protein delivery No charge.
  • Guaranteed yield: 3-5 mg purified protein.
  • Guaranteed purity: >85% protein purity.
  • Guaranteed soluble protein: We prioritize proteins obtained from supernatant.
  • Competitive price: starting from $1,550
  • Full additional service: including endotoxin removal, tag removal, 13C & 15N labeling, and large scale production.

Service Content

Project process Timeline Price Deliverables
Codon optimization 2-3 weeks $0.3/bp
  • Cloning vector
  • 3~5 mg purified protein
  • QC data and final report
Gene synthesis and vector construction
Transformation 3-4 weeks

$1,550

Expression optimization
Affinity/multiple-step purification
QC (SDS-PAGE and WB)

Related Services

  • Custom production of highly-specific antibodies with high ELISA titer.
  • Hybridoma preparation.
  • Biomolecular interaction analysis.
  • Extended analytics services such as mass spectrometry, endotoxin detection, MALDI-TOF.

bacterial protein expression service process

Step 1: Gene synthesis

Our in-house bioinformatics tool translates the same amino acid to multiple triplet codons. This enhances recombinant protein expression by plotting and adjusting the codon usage frequency and by opening up stem-loops in the process of transcription and translation, and subsequently increases E.coli protein production yield by extending mRNA stability.

Codon optimization
codon optimization case study
mRNA structure optimization
mrna structure optimization case study
Fig. 1: Codon and mRNA structure optimization in the process of gene synthesis

Step 2: Protein expression

Using our state-of-the-art technologies, we have established rapid E.coli protein production platforms which ensure high yield delivery of the desired protein while offering our clients a lot of flexibility.

e.coli protein expression - case study
Fig. 2: SDS-PAGE analysis of target protein V1 expression

Lane M: Protein marker
Lane 0: Cell lysate without induction
Lane 1: Cell lysate with IPTG induction at 15°C, overnight
Lane 2: Cell lysate with IPTG induction at 37°C, 4h

Step 3: Protein purification

Our optimized purification methods minimize contaminants and cut down the number of steps to obtain the maximum amount of functional protein.

e.coli protein expression - case study
Fig. 3: SDS-analysis of target protein V1 in whole cell lysis by affinity chromatography

Lane M: Protein Marker
Lane 1: Supernatant after centrifugation
Lane 2: Flow through
Lane 3-12: 50 mM imidazole eluted fractions
Lane 13-22: 100 nM imidazole eluted fractions
Lane 23-26: 300 nM imidazole eluted fractions

Your Concerns

  1. I haven't had much success performing E.coli protein production. Could you please give me your professional advice?
    The following elements should be taken into consideration:

    • Your gene of interest is not compatible with E.coli protein expression systems. Differences in codons, mRNA structures, and transmembrane domains might be hindering the expression.
    • E.coli protein expression conditions such as temperature, hosts, vectors, and chaperones have not been optimized.
    • Your protein of interest may form inclusion bodies in the process of E.coli protein production. Refolding in moderate conditions favors proper folding and maximizes protein activity.

    BiologicsCorp offers mature flexible solutions to solve problems in E.coli protein expression. Please feel free to contact us if you need any help with your project.

  2. What do I need to provide for your E.coli protein expression services?
    All you need to do is tell us your target gene or protein sequences. In addition, attach details of your previous experience or references if possible. Our professional PhD-level teams will analyze the protein properties and design a strategy for you. Your project will be initiated after the approval of the strategy by both parties.

Contact Us

phone+1 (317) 703-0614
fax +1 (855) 427-1516
 For questions and/or a price quote, please inquire online or fill in a pre-order form.
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