BMP-2(Bone Morphogenetic Protein 2), Human Recombinant, E.coli

Full Name	Bone Morphogenetic Protein-2 Human Recombinant
Synonyms	BMP-2; BMP2; Bone Morphogenetic Protein 2; BMP2A
Source	E. coli
Amino Acid Sequence	The sequence of the N-terminal amino acids was determined as Met-Gln-Ala-Lys-His.
Molecular Weight	Bone Morphogenetic Protein-2 Human Recombinant was expressed in E. coli as a homodimeric, non-glycosylated polypeptide chain containing 115 amino acids.  BMP2 exhibits a Molecular Weight of approximately 26 kDa  as revealed by SDS-PAGE (under non-reducing conditions).
Purity	>98% as determined by SDS-PAGE and HPLC
Endotoxin	Endotoxin level was found to be <0.1 ng/µg(1EU/µg) by a LAL gel clot method.
Formulation	Lyophilized from a 50mM acetic acid solution, filtered by 0.2 µm membrane.
Reconstitution	To reconstitute the protein, add sterile 100mM AcOH (acetic Acid) to the lyophilized protein and make a preparation with a concentration ≥0.1mg/ml.  The preparation can be diluted into other appropriate buffers if necessary. Cautions should be taken to avoid bacterial growth, resulting in potential contamination with endotoxin.
Stability	The lyophilized protein is stable at room temperature (≈3 weeks) and at -20°C (≥ 2 years from the date of receipt).Upon reconstitution, it can be stored at 4°C (≤ 7 days) or at -20°C (≤ 6 months). For long term storage, please store at -80°C and the addition of a carrier protein (0.1% HSA or BSA) is recommended.Avoid frequent freeze-thaw cycles.
Biological Activity	Determined by its ability to induce alkaline phosphatase production in ATDC-5 cells. The ED50 is <50ng/ml.
Usage	FOR LABORATORY RESEARCH USE ONLY.  Not intended for diagnostic or therapeutic purposes.

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