Gene Knock-out Cell Line Service

CRISPR genes belong to a special DNA repeat sequences family that is ubiquitous in bacterial and archaeological genome. As a “genetic weapon” to defend against foreign genetic material, it can identify and cut foreign DNA to silent their expression. Due to its precise targeting, CRISPR system has been developed as a gene editing tool with high efficiency.

crispr-gene

Biologics International Corp (abbr. BIC) offers gene knock-out services. We can knock-out any gene of interest in the cell’s genome. You only need to provide the target gene sequence, we can provide you with cost-effective gene knockout service, periodic progress updates, and transparent service process to solve any of your concerns.

Key Features

  • High success rate, low non-specific target rate: multi gRNA design and verification, with non-specific target analysis and testing services
  • Rapid delivery with full transparency: delivery knockout cell lines in ~3 months, periodic progress report, transparent during the whole process
  • One-stop service: We focuses on the study of gene and recombinant protein. We provide one-stop service from gRNA design to protein detection (optional)

Service Process

  • Cell preparation:
  • Based on the cell type required, we prepare cells two weeks in advance to maintain the high activity of cells

  • Identify target knockout gene:
  • Identify CDS region of the gene, and analyze the corresponding genomic structure to determine the exon part of CDS. Based on the nature of gene, candidate knockout sites are selected and then determined

  • gRNA design and vector construction:
  • 3-5 groups of gRNA will be designed and the corresponding plasmids will be constructed which also integrate Cas9

  • Cell transfection and obtaining monoclonal cells:
  • The constructed plasmids are transfected into the host cell to generate cell pool. We will sequence the cells in the cell pool to verify the knock out effect

  • Screening for positive clones:
  • Using limited dilution method, transfected cells are plated on 96-well plate for monoclonal screening. Positive monoclonal cells will be amplified, sequenced, identified and stored. At this end, the cell line has been successfully constructed

  • Stability test (to be updated)
  • Mycoplasma detection:
  • Q-PCR and Western Blot

  • Cell line frozen and delivery

Contact Us

phone+1 (317) 703-0614
fax +1 (855) 427-1516
 For quotations, please use our online form, or fill the quotation form and email to Sales@BiologicsCorp.com.
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