BiologicsCorp (Abbr. BIC) offers bacterial fermentation services to produce industry-scale protein from batch, fed-batch and continuous cultures up to 500 liters of bacteria. Through a variety of optimization techniques including codon, pilot expression and fed-batch fermentation optimization as well as purification optimization, BIC can offer optimal solution for high level production of recombinant proteins with guaranteed purity.
|Characteristics||Standard fermentation||High cell density fermentation||Process optimization|
|Wet cell weight(g/L)||7-17||23-130||23-130|
|Expected protein final yield(mg/L)||5-50||20-400||100-1000|
|Timeline||1 week||3 weeks||8-12 weeks|
|Price||Start from $390||Start from $690||Please inquire|
The high cell density fermentation by fed-batch strategies is one of the most cost-effective means of achieving high yields for the production of heterologous proteins, which is widely used in the bio-industry. In fed-batch cultures, cell mass and productivity are maximized by controlling culture conditions such as the temperature and pH, the composition of the feed media, and the substrate feed rate.
(A) Shake flask and low cell density processes.
(B) High cell density processes.
Two benchtop fermentors configured with five-liter vessels are available for comparative studies. Batch, fed-batch and continuous cultures of 5 to 500 liters of yeast, bacteria are produced under stringently controlled conditions using four state-of-the-art fermentors.
At the pilot scale, two 100-liter fermentors and one 500-liter fermentor are available. Constant monitoring of each run ensures the pH, temperatures, aeration, dissolved oxygen content and agitation rate can be stringently controlled. As a result we are able to accurately reproduce growth conditions to provide consistency in the quality of product produced. Support equipments include shaker incubators, laminar flow hoods, chemical hoods, clean steam, microscopes, autoclaves, cryostorage, and freeze-dryers.
It is accessible to add nutrients or induction chemicals at any point during a run. Addition of nutrient and components during the run allows fed-batch and induction experiments to be performed. Optimization experiments can also be achieved, as regular sampling through a sterile sample port is possible during the fermentation.
Quality control is maintained by regular vessel sampling, and metabolite testing ensures the final product meets your specification. The product can be sent as a frozen cell pellet or further processed to produce concentrated protein extracts.