Cell-based recombinant protein expression and purification systems are the oldest and most widely used protein expression systems in the biotechnology industry with bacteria, mammalian, insect, and yeast cells featuring among the most commonly used host systems.
BIC (BiologicsCorp) provides high-quality cost-effective recombinant protein expression services in a timely manner. Our comprehensive gene-to-protein/antibody platform combines over ten years of expertise in gene synthesis, protein expression, process optimization, antibody production, and flexible solutions to provide researchers around the globe with high activity proteins tailored to their specific needs. We also offer tag-free protein production, endotoxin removal, and high-throughput protein production services.
|Characteristics||E. coli||Yeast||Insect cells||Mammalian cells|
|Turnaround Time||4~6 weeks||6~7 weeks||7~8 weeks||7~9 weeks|
|Expression level||High||Low – high||Low – high||Low – moderate|
|Secretion to periplasm||Secretion to medium||Secretion to medium||Secretion to medium|
|Protein folding||Partial folding||Proper folding||Proper folding||Proper folding|
Our in-house bioinformatics tool translates the same amino acid to multiple triplet codons, This enhances recombinant protein expression by plotting and adjusting the codon usage frequency and by opening up stem-loops in the process of transcription and translation.More about codon and mRNA structure optimization
We provide nickel affinity purification by default. Ion exchange purification, size-exclusion chromatography, and hydrophobic chromatography are also available. You can also provide customized protocols to optimize your project. More about protein purification
We possess state-of-the-art protein refolding technologies for recombinant protein production such as dialysis, dilution, and on-column refolding. By using multi-well plates to explore refolding conditions, our team can determine the optimal solution for stable and uniformly refolded proteins with good solubility. More about protein refolding
A multitude of techniques such as MALDI-TOF MS, HPLC and N-terminal sequencing are used for protein identification and characterization. We are committed to manufacture and develop high-quality protein products by combining rigorous process development with the most advanced protein identification techniques. More about protein identification and characterizartion
If you need help with system selection, our technical support will be happy to work with you to determine the best solutionfor your project. In brief, bacterial protein expression systems are the most rapid and cost-effective method for obtaining high-level expression. Proteins produced in mammalian cells have the advantage of going through complete post-translational modifications. However,mammalian cell systems are costly and present major difficulties in scaling up production.
We have delivered purified proteins to world wide customers with a success rate of over 95%. Our large collection of in-house technologies and flexible solutions enables us to complete very challenging projects. Nonetheless, it is best to keep the target proteins between 10kDa to 150kDa. Also, signal peptides and multiple transmembrane domains should be removed for improved expression yield.