Protein Expression Services—BiologicsCorp

Cell-based recombinant protein expression and purification systems are the the most classic and widely used protein expression systems in the biotechnology industry with bacteria, mammalian, insect, and yeast cells featuring among the most commonly used host systems.
BIC (BiologicsCorp) provides high-quality and cost-effective recombinant protein expression services in a timely manner. Our comprehensive gene-to-protein/antibody platform combines over ten years of expertise in gene synthesis, protein expression, process optimization, antibody production, and flexible solutions to provide researchers around the globe with high activity proteins tailored to their specific needs.

BIC (BiologicsCorp) possesses comprehensive services for recombinant protein production and its corresponding downstream applications, including industrial-scale protein production, antigen preparation, protein-protein interaction identification, etc.

Custom Antibody Service

One-stop service from gene to antibody, ELISA titer up to 1:512,000, positive WB Guaranteed.More>>

Large Scale Production Service

Deliverable 23~130 g/L of cell wet weight or 20-400 mg/L of customer specified proteinMore>>

Protein Interaction Assay

ForteBio biomolecular interaction analysis, wide range of biomolecular interactions.More>>

Recombinant Protein Service Technology Platforms

custom protein expression with codon optimization

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Codon and mRNA structure optimization

Our in-house bioinformatics tool translates the same amino acid to multiple triplet codons, This enhances recombinant protein expression by plotting and adjusting the codon usage frequency and by opening up stem-loops in the process of transcription and translation.More about codon and mRNA structure optimization

protein purification, protein purification service

Multiple purification methods

We provide nickel affinity purification by default. Ion exchange purification, size-exclusion chromatography, and hydrophobic chromatography are also available. You can also provide customized protocols to optimize your project. More about protein purification

protein refolding

Multi-channel protein refolding

We possess state-of-the-art protein refolding technologies for recombinant protein production such as dialysis, dilution, and on-column refolding. By using multi-well plates to explore refolding conditions, our team can determine the optimal solution for stable and uniformly refolded proteins with good solubility. More about protein refolding

Protein identification

Protein identification and characterization

A multitude of techniques such as MALDI-TOF MS, HPLC and N-terminal sequencing are used for protein identification and characterization. We are committed to manufacture and develop high-quality protein products by combining rigorous process development with the most advanced protein identification techniques. More about protein identification and characterizartion

Customer Concerns

What type of recombinant protein expression system should I choose?

If you need help with system selection, our technical support will be happy to work with you to offer professional opinions for your project. In brief, bacterial protein expression systems are the most rapid and cost-effective method for obtaining protein with high-level expression. Proteins produced in mammalian cells have the advantage of going through complete post-translational modifications. However, mammalian cell systems are costly and present major difficulties in scaling up production.

Selection guide of recombinant protein expression systems

Characteristics E. coli Yeast Insect cells Mammalian cells
Turnaround Time 4~6 weeks 6~7 weeks 7~8 weeks 7~9 weeks
Price Low Low High High
Expression level High Low – high Low – high Low – moderate
Extracellular
expression
Secretion to periplasm Secretion to medium Secretion to medium Secretion to medium
Protein folding Partial folding Proper folding Proper folding Proper folding
Post-translational
modifications
Poor Moderate Moderate Comprehensive
Advantages
  • Rapid expression
  • Low cost
  • Scaleable
  • Well-characterized genetics
  • Moderately rapid expression
  • Efficient protein folding
  • Relatively low cost
  • Scaleable
  • Good expression levels for intracellular proteins
  • Relatively rapid growth
  • Efficient protein folding
  • Efficient protein folding
  • Comprehensive post-translational modifications
Disadvantages
  • Poor folding
  • Inclusion body formation
  • Minimal post-translational modifications
  • Different glycosylation
  • Tendancy to form hyperglycosylated proteins
  • N-glycan structures considered allergenic
  • Expensive culture media
  • Large volumes of virus needed on scale-up
  • Different Glycosylation and N-linked glycan structures
  • Expensive culture media
  • Difficult to scale-up

Is there any limit on the products in your protein production services?

We have delivered purified proteins to worldwide customers with a success rate of over 95%. Our large collection of in-house technologies and flexible solutions enables us to complete very challenging projects. Nonetheless, it is best to keep the target proteins between 10kDa to 150kDa. Also, signal peptides and multiple transmembrane domains should be removed for improved expression yield

Contact With Our Scientific Experts

We would be happy to provide you with support on your protein research project. Let us know your questions and requests and our scientific experts will reply shortly.





  +1 (317) 703-0614
  +1 (855) 427-1516

* To get a price quotation, please fill the protein service pre-order form and email to Sales@BiologicsCorp.com.