Cell-based recombinant protein expression and purification systems are the the most classic and widely used protein expression systems in the biotechnology industry with bacteria, mammalian, insect, and yeast cells featuring among the most commonly used host systems.
BIC (BiologicsCorp) provides high-quality and cost-effective recombinant protein expression services in a timely manner. Our comprehensive gene-to-protein/antibody platform combines over ten years of expertise in gene synthesis, protein expression, process optimization, antibody production, and flexible solutions to provide researchers around the globe with high activity proteins tailored to their specific needs.
BIC (BiologicsCorp) possesses comprehensive services for recombinant protein production and its corresponding downstream applications, including industrial-scale protein production, antigen preparation, protein-protein interaction identification, etc.
Custom Antibody Service
One-stop service from gene to antibody, ELISA titer up to 1:512,000, positive WB Guaranteed.More>>
Large Scale Production Service
Deliverable 23~130 g/L of cell wet weight or 20-400 mg/L of customer specified proteinMore>>
Protein Interaction Assay
ForteBio biomolecular interaction analysis, wide range of biomolecular interactions.More>>
Our in-house bioinformatics tool translates the same amino acid to multiple triplet codons, This enhances recombinant protein expression by plotting and adjusting the codon usage frequency and by opening up stem-loops in the process of transcription and translation.More about codon and mRNA structure optimization
We possess state-of-the-art protein refolding technologies for recombinant protein production such as dialysis, dilution, and on-column refolding. By using multi-well plates to explore refolding conditions, our team can determine the optimal solution for stable and uniformly refolded proteins with good solubility. More about protein refolding
A multitude of techniques such as MALDI-TOF MS, HPLC and N-terminal sequencing are used for protein identification and characterization. We are committed to manufacture and develop high-quality protein products by combining rigorous process development with the most advanced protein identification techniques. More about protein identification and characterizartion
If you need help with system selection, our technical support will be happy to work with you to offer professional opinions for your project. In brief, bacterial protein expression systems are the most rapid and cost-effective method for obtaining protein with high-level expression. Proteins produced in mammalian cells have the advantage of going through complete post-translational modifications. However, mammalian cell systems are costly and present major difficulties in scaling up production.
|Characteristics||E. coli||Yeast||Insect cells||Mammalian cells|
|Turnaround Time||4~6 weeks||6~7 weeks||7~8 weeks||7~9 weeks|
|Expression level||High||Low – high||Low – high||Low – moderate|
|Secretion to periplasm||Secretion to medium||Secretion to medium||Secretion to medium|
|Protein folding||Partial folding||Proper folding||Proper folding||Proper folding|
We have delivered purified proteins to worldwide customers with a success rate of over 95%. Our large collection of in-house technologies and flexible solutions enables us to complete very challenging projects. Nonetheless, it is best to keep the target proteins between 10kDa to 150kDa. Also, signal peptides and multiple transmembrane domains should be removed for improved expression yield
We would be happy to provide you with support on your protein research project. Let us know your questions and requests and our scientific experts will reply shortly.