Our biomolecular interaction analysis service is based on a Bio-Layer Interferometry (BLI) technology developed by ForteBio, capable of label-free detection and monitoring biomolecular interactions in real time. A huge array of biomolecular interactions can be studied, such as protein protein interactions, small molecule and protein interactions. With a strong expertise in protein science and years of experience offering flexible solutions to a world wide clientele, BiologicsCorp guarantees the delivery of very accurate analysis of your samples following work plans tailored to your specific needs.
BIC’s biomolecular interaction analysis service can measure wide range of biomolecular interactions including protein to protein, DNA to protein, metabolite to protein, antibody to antigen, enzyme to substrate/inhibitor, and small molecule to drug target interactions.
|Sample preparation (optional)*||4-6 weeks||Please inquire|
|Antigen and antibody fragment characterization||
|Protein-protein/ peptide interactions||
|Protein-small molecule interactions||
*: We provide a one-stop biomolecular interaction analysis service starting from custom protein/ antibody production, allowing us to better assist our customers in preparing high quality samples suited for their intended application.
Bio-layer interferometry is a well-established optical label-free technique to study biomolecular interactions. In Octet® RED96 system, the interaction occurs at the very tip of a glass fiber-based BLI biosensor. White light passing through the glass fiber is reflected back from both the internal surface and the external interface (with the sample solution). As more molecules bind to the sensor, the thickness variation of the external layer of immobilized protein causes the interference pattern shifts. The pattern can be plotted as a nanometer shift of relative intensity vs. wavelength that can be recorded in real-time with high precision and accuracy.
As a promising biosensor platform, BLI meets the needs of fields as diverse as biological research, biotherapeutic and small molecule drug development research, immunogenicity studies and vaccine development, and QC.
Verzijl D, Riedl T, Parren PW, Gerritsen AF. A Novel Label-free Cell-based Assay Technology Using Biolayer Interferometry. Biosens Bioelectron. 2016 Aug 28:87:388-395.
Sun Y, Xiao S, Chen J, Wang M, Zheng Z, Song S, Zhang L. Heat Shock Protein 90 Mediates the Apoptosis and Autophage in Nicotinic-Mycoepoxydiene-Treated HeLa Cells. Acta Biochim Biophys Sin (Shanghai). 2015 Jun: 47(6):451-8.
Shah NB, Duncan TM. Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects. J Vis Exp. 2014 Feb 18: (84):e51383.
|No.||Conc.(nM)||KD (M)||KD Error||kon(1/Ms)||kon Error||koff(1/s)||Full R^2|
The immobilized ligand interacts strongly with the analyte in solution, as KD values of different concentrations are less than 1.0E-12.
Please feel free to contact us regarding your projects and we will be pleased to provide related information. We guarantee an accurate analysis of your samples following work plans tailored to your needs.
To get a price quotation, please fill Biomolecular Interaction Analysis Service Quote Form and email to Sales@BiologicsCorp.com.