IGF1 (Insulin-like Growth Factor 1), Human Recombinant, E.coli

Full Name	Insulin-Like Growth Factor 1 Human Recombinant
Synonyms	Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA, Mechano growth factor, MGF.
Source	E. coli
Molecular Weight	Recombinant Human IGF-I produced by e.coli expression system.Target protein is expressed with sequence (L53-A118) of Human IGF1.(Uniprot #P05019). Insulin-Like Growth Factor-I Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 65 amino acids and having a MW of 7.5KD
Purity	>95% as determined by SDS-PAGE and HPLC
Endotoxin	Endotoxin level was found to be ≤ 0.1 ng/µg(1EU/µg) by LAL gel clot method.
Formulation	Lyophilized from a 0.2µm filtered solution of 300mM NaAc, pH 6.5.
Reconstitution	To reconstitute the protein, add sterile water to the lyophilized protein and make a preparation with a concentration ≥0.1mg/ml. The preparation can be diluted into other appropriate buffers if necessary. Be cautious and avoid potential contamination with bacteria and endotoxin
Stability	The lyophilized form of the protein is stable at room temperature for 3 weeks and at -20°C for at least 2 years from the date of receipt. Upon reconstitution, the protein should be stored at 4°C for no more than 7 days. The protein is stable at -20°C for six months. For long term storage, please store at -80°C and the addition of a carrier protein (0.1% HSA or BSA) is recommended. Please prevent freeze-thaw cycles.
Biological Activity	The ED50, calculated by the dose-dependant proliferation of murine BALB\C 3T3 cells (measured by 3H-thymidine uptake) is < 1.0ng/ml, corresponding to a specific activity of 1,000,000U/mg. For most in-vitro applications, IGF-I exerts its biological activity in the concentration range of 0.2-20 ng/ml.
Usage	LABORATORY RESEARCH USE ONLY. Not intended for diagnostic or therapeutic purposes.
Reference	Rosete Sofía Pais, Nuria Moreno-Barriuso. “Transcriptome Analysis in Prenatal IGF1-Deficient Mice Identifies Molecular Pathways and Target Genes Involved in Distal Lung Differentiation” Elias Stratikopoulos, Matthias Szabolcs and Argiris Efstratiadis. “The hormonal action of IGF1 in postnatal mouse growth” Sarah F. Bortvedt and P. Kay Lund. “Insulin-like growth factor 1: common mediator of multiple enterotrophic hormones and growth factors”

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