eIF4E(Phospho-Ser209) Polyclonal Antibody

Description	Rabbit Polyclonal
Synonyms	mRNA cap-binding protein; eIF-4F 25 kDa subunit;
Formulation	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Application	WB IHC IF
Application Details	Predicted MW: 25kd Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:200
Specificity	The antibody detects endogenous level of eIF4E only when phosphorylated at serine 209.
Immunogen	Peptide sequence around phosphorylation site of serine 209 (S-G-S(p)-T-T) derived from Human eIF4E.
Key Properties	Hu The antibody detects endogenous level of eIF4E only when phosphorylated at serine 209. Peptide sequence around phosphorylation site of serine 209 (S-G-S(p)-T-T) derived from Human eIF4E.
Purifiction	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Species Reactivity	Hu
Storage	Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

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