IFN-alpha 2b | Interferon-Alpha 2b, human

Full Name	Interferon-Alpha 2b Human Recombinant
Synonyms	Interferon alpha 2b, IFNA, INFA2, MGC125764, MGC125765.
Source	E. coli
Amino Acid Sequence	The sequence of the N-terminal amino acids was determined to be Met-Cys-Asp-Leu-Pro.
Molecular Weight	Interferon-a 2b Human Recombinant was produced in E. coli as a single, non-glycosylated, polypeptide chain containing 166 amino acids and shown to exhibit a molecular mass of ≈19.4 kDa by SDS-PAGE.
Purity	>95% as determined by SDS-PAGE and HPLC
Endotoxin	Endotoxin level was found to be ≤ 0.1 ng/µg(1EU/µg) by a LAL gel clot method.
Formulation	Lyophilized from a sodium phosphate dibasic (2.3 mg) and sodium phosphate monobasic (0.55 mg) buffer, filtered with a membrane (0.2 µm).
Reconstitution	To reconstitute the protein, add sterile water to the lyophilized protein and make a preparation with a concentration ≥0.1mg/ml. The preparation can be diluted into other appropriate buffers if necessary. Cautions should be taken to avoid bacterial growth, resulting in potential contamination with endotoxin.
Stability	Once lyophilized, the protein is stable at room temperature (≈3 weeks) and at -20°C (≥ 2 years from the date of receipt).Upon reconstitution, it can be stored at 4°C (≤ 7 days) or at -20°C (≤ 6 months). For long term storage, please store at -80°C and the addition of a carrier protein (0.1% HSA or BSA) is recommended.Avoid frequent freeze-thaw cycles.
Biological Activity	In a viral resistance assay using bovine kidney MDBK cells, the specific activity was determined to be 200,000,000 IU/ mg.
Usage	FOR LABORATORY RESEARCH USE ONLY. Not intended for diagnostic or therapeutic purposes.

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