Insulin-Like Growth Factor Binding Protein-3 Human Recombinant
IGFBP3 is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
| Description | IGFBP3 Human Recombinant produced in E.Coli is a homodimeric, non-glycosylated, polypeptide chain containing 2x264 amino acids and having a molecular mass of 28806 Dalton. IGFBP-3 is purified by proprietary chromatographic techniques. |
| Synonyms | Growth-hormone-dependant binding protein, IBP3, BP-53, IGFBP-3. |
| Source | Escherichia Coli. |
| Amino Acid Sequence | GASSGGLGPV VRCEPCDARA LAQCAPPPAV CAELVREPGC GCCLTCALSE GQPCGIYTER CGSGLRCQPS PDEARPLQAL LDGRGLCVNA SAVSRLRAYL LPAPPAPGNA SESEEDRSAG EVESPSVSST HRVSDPKFHP LHSKIIIIKK GHAKDSQRYK VDYESQSTDT QNFSSESKRE TEYGPCRREM EDTLNHLKFL NVLSPRGVHI PNCDKKGFYK KKQCRPSKGR KRGFCWCVDK YGQPLPGYTT KGKEDVHCYS MQSK. |
| Purity | >96% as determined by SDS-PAGE and HPLC |
| Formulation | Sterile Filtered White lyophilized (freeze-dried) powder. Lyophilized from a 0.2µm filtered concentrated (0.5mg/ml) solution in PBS, pH 7.4. |
| Stability | The lyophilized form of the protein is stable at room temperature for 3 weeks and at -20°C for at least 2 years from the date of receipt. Upon reconstitution, the protein should be stored at 4°C for no more than 7 days. The protein is stable at -20°C for six months. For long term storage, please store at -80°C and the addition of a carrier protein (0.1% HSA or BSA) is recommended. Please prevent freeze-thaw cycles. |
| Biological Activity | The ED50, calculated by by its ability to inhibit IGF-II induced proliferation of MCF-7 is < 0.2 µg/ml in the presence of 15 ng/ml of Human IGF-II, corresponding to a specific activity of 5,000,000units/mg. |
| Solubility | It is recommended to reconstitute the lyophilized Insulin-Like Growth Factor Binding Protein-3 in sterile 20mM AcOH (acetic Acid) not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Usage | LABORATORY RESEARCH USE ONLY. Not intended for diagnostic or therapeutic purposes. |
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Product Code: GF357
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