IL-22 | Interleukin-22, mouse

Full Name	Interleukin-22 Mouse Recombinant
Synonyms	IL-TIF, TIFa, IL-10-related T-cell-derived-inducible factor, IL-22, ILTIF, IL-D110, zcyto18, MGC79382, MGC79384, TIFIL-23.
Source	E. coli
Amino Acid Sequence	The sequence of the N-terminal amino acids was determined to be Met-Leu-Pro-Val-Asn.
Molecular Weight	Interleukin-22 Mouse Recombinant was produced in E. coli as a single, non-glycosylated homodimeric polypeptide chain containing 147 amino acids and shown to exhibit a molecular mass of 16.7 kDa.
Purity	>95% as determined by SDS-PAGE and HPLC
Endotoxin	Endotoxin level was found to be ≤ 0.1 ng/µg(1EU/µg) by a LAL gel clot method.
Formulation	Lyophilized from a PBS solution pH7.4, filtered with a membrane (0.2 µm).
Reconstitution	To reconstitute the protein, add sterile water to the lyophilized protein and make a preparation with a concentration ≥0.1mg/ml. The preparation can be diluted into other appropriate buffers if necessary. Cautions should be taken to avoid bacterial growth, resulting in potential contamination with endotoxin.
Stability	Once lyophilized, the protein is stable at room temperature (≈3 weeks) and at -20°C (≥ 2 years from the date of receipt).Upon reconstitution, it can be stored at 4°C (≤ 7 days) or at -20°C (≤ 6 months). For long term storage, please store at -80°C and the addition of a carrier protein (0.1% HSA or BSA) is recommended.Avoid frequent freeze-thaw cycles.
Biological Activity	The ED50, as determined by its ability to induce IL-10 secretion in Colo205 cells, is ≤ 0.5ng/ml, corresponding to a Specific Activity of 2MIU/mg.
Usage	FOR LABORATORY RESEARCH USE ONLY. Not intended for diagnostic or therapeutic purposes.

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