IFN gamma Human
The major interferon produced by mitogenically or antigenically stimulated lymphocytes. It is structurally different from type I interferon and its major activity is immunoregulation. It has been implicated in the expression of class II histocompatibility antigens in cells that do not normally produce them, leading to autoimmune disease. Interferon gamma is produced mainly by T-cells and natural killer cells activated by antigens, mitogens, or alloantigens. It is produced by lymphocytes expressing the surface antigens CD4 and CD8. The synthesis of IFN-gamma is induced, among other things, by Interleukin-2, FGF-basic, and EGF.
| Full Name | Interferon-Gamma Human Recombinant |
| Synonyms | Immune Interferon, type II interferon, T cell interferon, MAF, IFNG, IFG, IFI, IFN-gamma. . |
| Source | E. coli |
| Amino Acid Sequence | The sequence of the first fifteen N-terminal amino acids was determined and was found to be Gln-Asn-Pro-Tyr-Val-Lys-Glu-Ala-Glu-Asn-Leu-Lys-Lys- Tyr-Phe. |
| Molecular Weight | Recombinant Human Interferon gamma produced in E. coli is a single, non-glycosylated, polypeptide chain containing 144 amino acids and having a molecular mass of 17kDa. |
| Purity | >95% as determined by SDS-PAGE and HPLC |
| Endotoxin | Endotoxin level was found to be ≤ 0.1 ng/µg(1EU/µg) by LAL gel clot method. |
| Formulation | Lyophilized froma 0.2µm filtered solution of 20mM PB,150mM NaCl,pH7.0. |
| Reconstitution | To reconstitute the protein, add sterile water to the lyophilized protein and make a preparation with a concentration ≥0.1mg/ml. The preparation can be diluted into other appropriate buffers if necessary. Be cautious and avoid potential contamination with bacteria and endotoxin |
| Stability | The lyophilized form of the protein is stable at room temperature for 3 weeks and at -20°C for at least 2 years from the date of receipt. Upon reconstitution, the protein should be stored at 4°C for no more than 7 days. The protein is stable at -20°C for six months. For long term storage, please store at -80°C and the addition of a carrier protein (0.1% HSA or BSA) is recommended. Please prevent freeze-thaw cycles. |
| Biological Activity | The specific activity as determined in a viral resistance assay using VSV-WISH cells was found to be greater than 1.5 x 107 IU/ mg. |
| Usage | LABORATORY RESEARCH USE ONLY. Not intended for diagnostic or therapeutic purposes. |
Write a review
Your Name:Your Review: Note: HTML is not translated!
Rating: Bad Good
Enter the code in the box below:
Product Code: CY314
Availability: In Stock
Availability: In Stock
Price: $0.00
Ex Tax: $0.00
Ex Tax: $0.00
- OR -
