Frizzled-1 Human Recombinant

“Frizzled” gene family members encode 7-transmembrane domain proteins which are receptors for Wnt signaling proteins. The FZD1 protein includes a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, 7 transmembrane domains, and a C-terminal PDZ domain-binding motif. The FZD1 transcript is expressed in the adult heart, placenta, lung, kidney, pancreas, prostate, and ovary and in the fetal lung and kidney.
Description Frizzled-1 Human Recombinant produced in HEK293 cells, corresponding to the extracellular region of mature Human Frizzled-1 (amino acids 112-228) and fused to the Fc region of Human IgG1, having an MW of 46kDa.
Frizzled-1 is purified by proprietary chromatographic techniques.
Synonyms Frizzled-1, Fz-1, hFz1, FzE1, FZD1, FLJ95923, DKFZp564G072.
Source HEK293 (Human Embryonic Kidney cell line).
Amino Acid Sequence DHGYCQPISI PLCTDIAYNQ TIMPNLLGHT NQEDAGLEVH QFYPLVKVQC SAELKFFLCS MYAPVCTVLE QALPPCRSLC ERARQGCEAL MNKFGFQWPD TLKCEKFPVH GAGELCVENL YFQGGSGTKL DKTHTCPPCP APELLGGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHGD PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT LPPSRDELTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE ALHNHYTQKS LSLSPGK.
Purity >96% as determined by SDS-PAGE and HPLC
Formulation Sterile Filtered White lyophilized (freeze-dried) powder.
The protein was lyophilized without any additives.
Stability The lyophilized form of the protein is stable at room temperature for 3 weeks and at -20°C for at least 2 years from the date of receipt. Upon reconstitution, the protein should be stored at 4°C for no more than 7 days. The protein is stable at -20°C for six months. For long term storage, please store at -80°C and the addition of a carrier protein (0.1% HSA or BSA) is recommended. Please prevent freeze-thaw cycles.
Biological Activity The KD (equilibrium binding constant) of FZD1 Human was measured at less than 10nM with human Wnt3a as the ligand. The measurements were performed by the ForteBio Octet optical biosensor instrument.
Solubility It is recommended to reconstitute the lyophilized FZD1 in sterile 1xPBS not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Usage LABORATORY RESEARCH USE ONLY. Not intended for diagnostic or therapeutic purposes.

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Product Code: OT550
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