Cys-Protein G His Tag Recombinant

Description	Cys-Protein G His Tag Recombinant produced in E.Coli is a 202 amino acids protein which contains amino acid 190-384 of the Streptococcus sp with a 6-His tag Cysteine residue at the C-terminus, has a predicted molecular mass of approximately 22.5 kDa. But it migrates with an apparent molecular mass of 32kDa in SDS-PAGE. The Protein G His Tag is purified by proprietary chromatographic techniques.
Source	Escherichia Coli.
Purity	>96% as determined by SDS-PAGE and HPLC
Formulation	Sterile Filtered White lyophilized (freeze-dried) powder. Lyophilized with no additive.
Application	Protein G binds to the constant region of many species of immunoglobulin G. It can be used to detect, quantify and purify IgG antibodies and antibody/antigen complexes. Recombinant Protein G contains only IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains have been removed to ensure the maximum specific IgG binding capacity.
Stability	The lyophilized form of the protein is stable at room temperature for 3 weeks and at -20°C for at least 2 years from the date of receipt. Upon reconstitution, the protein should be stored at 4°C for no more than 7 days. The protein is stable at -20°C for six months. For long term storage, please store at -80°C and the addition of a carrier protein (0.1% HSA or BSA) is recommended. Please prevent freeze-thaw cycles.
Solubility	It is recommended to reconstitute the lyophilized Protein G in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Specificity	1. Binds with greater affinity to most mammalian immunoglobulins than Protein A, including human IgG3 and rat IgG2a. 2. Does not bind to human IgM, IgD and IgA.
Usage	LABORATORY RESEARCH USE ONLY. Not intended for diagnostic or therapeutic purposes.

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