Beta Lactamase Recombinant

Description	Beta-Lactamase TEM precursor Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 263 amino acids and having a molecular mass of 29 kDa. Beta Lactamase is purified by proprietary chromatographic techniques.
Synonyms	b-Lactamase, EC 3.5.2.6, TEM precursor.
Source	Escherichia Coli.
Amino Acid Sequence	MHPETLVK VKDAEDQLGA RVGYIELDLN SGKILESFRP EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL VEYSPVTEKH LTDGMTVRELCSAAITMSDN TAANLLLTTI GGPKELTAFL HNMGDHVTRL DRWEPELNEA IPNDERDTTM PAAMATTLRK LLTGELLTLA SRQQLIDWME ADKVAGPLLRSALPAGWFIA DKSGAGERGS RGIIAALGPD GKPSRIVVIY TTGSQATMDE RNRQIAEIGA SLIKHW.
Purity	>96% as determined by SDS-PAGE and HPLC
Formulation	Sterile Filtered White lyophilized (freeze-dried) powder. Lyophilized from a concentrated (1 mg/ml) solution in water containing 20mM Phosphate buffer pH-7.
Stability	The lyophilized form of the protein is stable at room temperature for 3 weeks and at -20°C for at least 2 years from the date of receipt. Upon reconstitution, the protein should be stored at 4°C for no more than 7 days. The protein is stable at -20°C for six months. For long term storage, please store at -80°C and the addition of a carrier protein (0.1% HSA or BSA) is recommended. Please prevent freeze-thaw cycles.
Solubility	It is recommended to reconstitute the lyophilized Beta Lactamase in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Specificity	700IU/mg.
Unit Definition	One unit will hydrolyze 1.0 μmole of indicated substrate per min at pH 7.0 at 25°C. The International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.
Usage	LABORATORY RESEARCH USE ONLY. Not intended for diagnostic or therapeutic purposes.

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