4E-BP1(Phospho-Thr45) Polyclonal Antibody
|Formulation||Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|Application Details||Predicted MW: 18kd
Western blotting: 1:500~1:1000
|Specificity||The antibody detects endogenous level of 4E-BP1 only when phosphorylated at Threonine 45.|
|Immunogen||Peptide sequence around phosphorylation site of threonine 45 (S-T-T(p)-P-G) derived from Human 4E-BP1.|
The antibody detects endogenous level of 4E-BP1 only when phosphorylated at Threonine 45.
Peptide sequence around phosphorylation site of threonine 45 (S-T-T(p)-P-G) derived from Human 4E-BP1.
|Purifiction||Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.|
|Storage||Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.|
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