BIC provides recombinant antibody production service. We express most varieties of recombinant antibody, including full-length antibody, chimeric antibody, scFv, Fab, Fab’ fragment, sdAb, di-scFv, Fc fusion protein, by using HEK293 and CHO cell.
BIC’s recombinant antibdy production service including antibody engineering and full-length antibody expression.
You only need to provide the antibody sequences. If you don’t have sequences, please contact us ,we will be happy to help you.
1.antibody fragment expression: scFv(di-scFv), sdAb, Fab fragment
2.IgG class switching: Chimeric antibody expression
We are able to change antibody from any species to any other species of IgG isotype
|Customer provide||Recombinant antibody production service||Deliverables||Timeline|
Recombinant antibody sequences
|Gene synthesis||Codon optimization||
|Antibody production||Recombinant antibody expression|
|Antibody purification||Protein A/G purification|
|QC||SDS-PAGE & Western blot|
* According to your requirements, we can provide different amounts of recombinant antibody expression : 1-10mg, 10-50mg, 50-100mg, >100mg recombinant antibody.
Codon optimization was run to remove potential negatively acting sequences and to modify codon usage in recombinant antibody production. High-yield expression achieved is attributable to sequence optimization, which leads to improved efficiency of mRNA translation.
To meet increasing demand on high quality recombinant antibody, we developed a novel vector with optimized promoter activity, which contributed to higher expression level compared with traditional CMV based vector.
Our increasing expression level of secreted recombinant antibody production is based on the self-developed mammalian dual expression vector. It comprises two different genes encoding two polypeptide chains (e.g. the heavy and light chains of target recombinant antibody). The method solves the problem of the plasmid with imbalanced heavy and light separately and leads to a considerable enhanced expression level. Host cell line that best fits the system is also available to improve recombinant antibody yield.
To request a quote, please fill in the recombinant antibody production service pre-order form and email it to email@example.com