Recombinant Antibody Production

Recombinant antibody production without use of animals. BIC provides recombinant antibody production service. We provides most varieties of recombinant antibody, including full-length antibody, chimeric antibody, scFv, Fab, Fab’ fragment, sdAb, di-scFv, Fc fusion protein.

What We Offer

BIC’s recombinant antibody production services include antibody engineering and full-length antibody expression.
You only need to provide the antibody sequences. All we need is your target antibody sequences.

recombinant antibody production
Full-length antibody expression

  • Recombinant antibody production: full-length antibody(recombinant IgG). we only need antibody sequences

recombinant Antibody expression
Antibody engineering

  • Antibody fragments: Fab, F(ab)’, sdAb, scFv, di-scFv and Fc fusion protein
  • Chimeric antibody: human-mouse chimeric antibody

*Antibody engineering:

  • antibody fragments expression: scFv(di-scFv), sdAb, Fab fragment
  • Chimeric antibody expression

We are able to change antibody from any species to any other species of IgG isotype  

Subtype:
Species:
recombinant antibody expression

 

Recombinant Antibody production Service

Customer provide Recombinant antibody production service Deliverables Timeline

Recombinant antibody sequences
Gene synthesis Codon optimization
  • 4ug gene in cloning vector
  • >90% purity antibody
  • Quantity as customer requirements
  • QC report
7-9weeks
Vector construction
Misc. Sub-cloning &
DNA max-prep
Antibody production Recombinant antibody expression
Antibody purification Protein A/G purification
QC SDS-PAGE & Western blot

Order Process

Order process

Self-developed high-level expression system

Codon optimization was run to remove potential negatively acting sequences and to modify codon usage in recombinant antibody production. High-yield expression achieved is attributable to sequence optimization, which leads to improved efficiency of mRNA translation.

  • Strong promoter

To meet increasing demand on high quality recombinant antibody, we developed a novel vector with optimized promoter activity, which contributed to higher expression level compared with traditional CMV based vector.

  • Dual-expression vector

Our increasing expression level of secreted recombinant antibody production is based on the self-developed mammalian dual expression vector. It comprises two different genes encoding two polypeptide chains (e.g. the heavy and light chains of target recombinant antibody). The method solves the problem of the plasmid with imbalanced heavy and light separately and leads to a considerable enhanced expression level. Host cell line that best fits the system is also available to improve recombinant antibody yield.

Key features of recombinant antibody production

  • High-specificity: bind to corresponding antigen specifically with high-affinity
  • Non-animal technology: alleviate animal welfare concerns
  • Fast speed: significantly shorter than conventional immune antibody production
  • Easy-to-manipulate: capable of guiding recombinant antibody production process
  • Isotype conversion: can be easily converted into any antibody isotype (e.g. IgA, IgM, and IgG etc.) from any species by adding the appropriate constant domain
  • Broad target antibody: antibodies to highly toxic or non-immunogenic antigens can be created
  • Amenable to high-throughput production: attractive to the field of proteomics

Real-time QC: antigen-antibody reaction analysis

The Octet® system (Octet® RED96) based on Bio-Layer Interferometry (BLI) was developed as a promising biosensor platform to detect binding specificity of interacting molecules, including recombinant antibody and corresponding antigens. Processed curves, KD value and other reaction rate constants will be provided after real-time, label-free measurements.
Real-time QC: antigen-antibody reaction analysis

Contact us

  +1 (317) 703-0614
  +1 (855) 427-1516

To request a quote, please fill in the recombinant antibody production service pre-order form and email it to sales@biologicscorp.com