TNF-alpha | Tumor Necrosis Factor-alpha, human

Full Name	Tumor Necrosis Factor-Alpha Human Recombinant
Synonyms	TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2.
Source	E. coli
Amino Acid Sequence	MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA E AKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL.
Molecular Weight	Tumor Necrosis Factor-α Human Recombinant was produced in E. coli as a single, non-glycosylated, polypeptide chain containing 158 amino acids (157 amino acids of the mature human TNF-alpha and an N-terminal methionine) and shown to have a molecular mass of 17.5 kDa.
Purity	>95% as determined by SDS-PAGE and HPLC
Endotoxin	Endotoxin level was found to be ≤ 0.1 ng/µg(1EU/µg) by a LAL gel clot method.
Formulation	Lyophilized from a 1xPBS filtered with a membrane (0.2 µm).
Reconstitution	To reconstitute the protein, add sterile water to the lyophilized protein and make a preparation with a concentration ≥0.1mg/ml. The preparation can be diluted into other appropriate buffers if necessary. Cautions should be taken to avoid bacterial growth, resulting in potential contamination with endotoxin.
Stability	Once lyophilized, the protein is stable at room temperature (≈3 weeks) and at -20°C (≥ 2 years from the date of receipt).Upon reconstitution, it can be stored at 4°C (≤ 7 days) or at -20°C (≤ 6 months). For long term storage, please store at -80°C and the addition of a carrier protein (0.1% HSA or BSA) is recommended.Avoid frequent freeze-thaw cycles.
Biological Activity	The Specific Activity is >5.0x107 IU/mg as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D.
Usage	FOR LABORATORY RESEARCH USE ONLY. Not intended for diagnostic or therapeutic purposes.

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