Smad3(Phospho-Ser425) Polyclonal Antibody

Description	Rabbit Polyclonal
Synonyms	JV15-2; MAD-3; MADH3; Mad3; Mothers against DPP homolog 3
Formulation	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Application	WB IHC
Application Details	Predicted MW: 52kd Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100
Specificity	The antibody detects endogenous level of Smad3 only when phosphorylated at serine 425.
Immunogen	Peptide sequence around phosphorylation site of serine 425 (C-S-S-V-S(p)) derived from Human Smad3.
Key Properties	Hu Ms Rt The antibody detects endogenous level of Smad3 only when phosphorylated at serine 425. Peptide sequence around phosphorylation site of serine 425 (C-S-S-V-S(p)) derived from Human Smad3.
Purifiction	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Species Reactivity	Hu Ms Rt
Storage	Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

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