LIF (Leukemia Inhibitory Factor), Human Recombinant, E.coli

Full Name	Leukemia Inhibitory Factor Human Recombinant
Synonyms	Leukemia Inhibitory Factor, LIF, CDF, HILDA, D-FACTOR, Differentiation- stimulating factor, Melanoma-derived LPL inhibitor, MLPLI, Emfilermin, DIA.
Source	E. coli
Amino Acid Sequence	The sequence of the N-terminal amino acids was determined to be Met- Ser-Pro-Leu-Pro.
Molecular Weight	Leukemia Inhibitory Factor (LIF) Human Recombinant was produced in E. coli as a single, non-glycosylated, polypeptide chain containing 180 amino acids and shown to exhibit a molecular mass of 19.7 kDa.
Purity	>95% as determined by SDS-PAGE and HPLC
Endotoxin	Endotoxin level was found to be ≤ 0.1 ng/µg(1EU/µg) by a LAL gel clot method.
Formulation	Lyophilized from a 1x PBS solution, filtered with a membrane (0.2 µm).
Reconstitution	To reconstitute the protein, add sterile water to the lyophilized protein and make a preparation with a concentration ≥0.1mg/ml. The preparation can be diluted into other appropriate buffers if necessary. Cautions should be taken to avoid bacterial growth, resulting in potential contamination with endotoxin.
Stability	Once lyophilized, the protein is stable at room temperature (≈3 weeks) and at -20°C (≥ 2 years from the date of receipt).Upon reconstitution, it can be stored at 4°C (≤ 7 days) or at -20°C (≤ 6 months). For long term storage, please store at -80°C and the addition of a carrier protein (0.1% HSA or BSA) is recommended.Avoid frequent freeze-thaw cycles.
Biological Activity	The ED50 was determined by the M1 cell differentiation assay to be < 0.01 ng/ml, corresponding to a specific activity of 100MIU/mg.
Usage	FOR LABORATORY RESEARCH USE ONLY. Not intended for diagnostic or therapeutic purposes.

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