CCL3/MIP 1a Human

Full Name	Macrophage Inflammatory Protein-1 Alpha Human Recombinant (CCL3)
Synonyms	Small inducible cytokine A3, CCL3, Macrophage inflammatory protein 1-alpha, MIP-1-alpha, Tonsillar lymphocyte LD78 alpha protein, G0/G1 switch regulatory protein 19-1, G0S19-1 protein, SIS-beta, PAT 464.1, chemokine (C-C motif) ligand 3, MIP1A, SCYA3, G0S19-1, LD78ALPHA.
Source	E. coli
Amino Acid Sequence	The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Asp-Thr-Pro-Thr.
Molecular Weight	Recombinant Human CCL3 produced in E. coli is a single, non-glycosylated, polypeptide chain containing 66 amino acids and having a molecular mass of 7.5 kDa.
Purity	Greater than 95% as determined by SEC-HPLC. Greater than 95% as determined by reducing SDS-PAGE
Endotoxin	Endotoxin level was found to be ≤ 0.1 ng/µg(1EU/µg) by LAL gel clot method.
Formulation	BIC recombinant CCL3/MIP-1 alpha is lyophilized from a 0.2µm filtered solution of 20mM PB,150mM NaCl, pH7.4.
Reconstitution	To reconstitute the protein, add sterile water to the lyophilized protein and make a preparation with a concentration ≥0.1mg/ml. The preparation can be diluted into other appropriate buffers if necessary. Be cautious and avoid potential contamination with bacteria and endotoxin
Stability	The lyophilized form of the protein is stable at room temperature for 3 weeks and at -20°C for at least 2 years from the date of receipt. Upon reconstitution, the protein should be stored at 4°C for no more than 7 days. The protein is stable at -20°C for six months. For long term storage, please store at -80°C and the addition of a carrier protein (0.1% HSA or BSA) is recommended. Please prevent freeze-thaw cycles.
Biological Activity	The ED50 of rHuCCL3 as determined by its ability to chemoattract human CCR5 transfected BaF3 mouse proB cells was 3 -10ng/mL
Usage	LABORATORY RESEARCH USE ONLY. Not intended for diagnostic or therapeutic purposes.

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