Transient transfection of mammalian cells expresses foreign DNA for a limited period time without integrating it into their genome and replicating new gene. As the most commonly used strategy for mammalian protein expression, transient transfection service provided by BiologicsCorp produce microgram quantities of high-quality proteins rapidly. The strategy has been applied to short-term transient expression of genes and small-scale protein production for research and development purpose.
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|Transient expression and purification||
Increasing stability of the transgene mRNA was proved to be key factors for high quality transient mammalian cell transfection. Stem-loop structure may encode features that cause mRNAs to be labile, which severely impact process of gene transcription and translation.
Our mRNA structure and codon optimization tools refer the same amino acid to multiple triplet codons. It also opens stem-loop structures in mRNA sequence and subsequently increase transient expression yield.
After transient expression, the optimized constructs achieved significantly increased high level expression compared with a previously wild type product.
Our in-house transfection reagent is designed to improve post transfection efficiency. It is formulated for transient transfection in mammalian expression system and generally presents significant advanced transfection efficiency than regular reagent.
In recent years, electroporation has become the preferred choice due to its high efficiency. The technique is a procedure gaining in popularity for gene transfer using high-voltage electric shocks to introduce DNA into cells. The DNA attached to feeder layer cells quickly and formed fast-growing colonies. The simple and efficient method yields a high frequency of protein transient expression.
(B)ControlOptimized strategy I
(C)ControlOptimized strategy II