BiologicsCorp offers risk free E.coli protein expression services for your research needs. With as little information as your gene name or protein sequences, our guaranteed service can deliver your protein of interest in the specified quantity and purity. To ensure a smooth and successful completion of your project, a dedicated PhD level technical support staff will be assigned to your project from initial inquiry to post-delivery. Our scientists will work closely with you to keep you apprised of project progress while taking all your goals into account. Bulk order discounts are also available.
Guaranteed quantity and purity: Get your desired quantity and purity or your money back.
Flexible solutions: Multiple approaches for assured success. How
BiologicsCorp offers time-tested flexible solutions for protein expression in E. coli. From codon optimization to small scale testing and scale up through protein purification and characterization, our professional teams use their extensive experience in protein expression to ensure the successful completion of every project. Thus far, our scientists have successfully delivered over a thousand recombinant proteins with a success rate above 95%.
Guaranteed soluble protein: You only get billed if solubility is achieved. How
We offer a soluble protein guarantee. Our proteins remain soluble even after repeated freeze-thaw cycles.
Maximum activity:We prioritize supernatant proteins. Why
Proteins secreted into the supernatant can maintain their native structure and function. We have established a comprehensive optimization platform for supernatant protein expression. In order to obtain proteins with the maximum activity, we prioritize supernatant proteins.
|Codon optimization||2-3 weeks||
|Gene synthesis and vector construction|
|QC (SDS-PAGE and WB)|
|Additional E. coli protein expression services:|
Our in-house bioinformatics tool translates the same amino acid to multiple triplet codons. This enhances recombinant protein expression by plotting and adjusting the codon usage frequency and by opening up stem-loops in the process of transcription and translation, and subsequently increases E.coli protein production yield by extending mRNA stability.
Using our state-of-the-art technologies, we have established rapid E. coli protein production platforms which ensure high yield delivery of the desired protein while offering our clients a lot of flexibility.
Lane M: Protein marker
Lane 0: Cell lysate without induction
Lane 1: Cell lysate with IPTG induction at 15°C, overnight
Lane 2: Cell lysate with IPTG induction at 37°C, 4h
Our optimized purification methods minimize contaminants and cut down the number of steps to obtain the maximum amount of functional protein.
Lane M: Protein Marker
Lane 1: Supernatant after centrifugation
Lane 2: Flow through
Lane 3-12: 50 mM imidazole eluted fractions
Lane 13-22: 100 nM imidazole eluted fractions
Lane 23-26: 300 nM imidazole eluted fractions
BiologicsCorp offers mature flexible solutions to solve problems in E. coli protein expression. Please feel free to contact us if you need any help with your project.