Endotoxin removal is an important part of recombinant protein expression and purification services as endotoxin-contaminated protein samples transfected into cells or injected into an animal host can initiate a strong immune response such as endotoxin shock, tissue injury, and even death. Endotoxin removal service of BiologicsCorp (Abbr. BIC) guarantees to effectively reduce the endotoxin level below 1 EU/µg throughout entire process and meet strict requirements for downstream animal and cell experiments.
BIC offers one-stop endotoxin removal service including gene synthesis, protein expression and purification, and endotoxin detection and removal. This service can be addressed in the flow chart below:
|Endotoxin Level Options||Timeline||Price($)||Deliverables|
|Additional 1-2 weeks||Start from 300$||
|<0.1 EU/µg||Please inquire|
Notes: we can effectively reduce the endotoxin level below 1 EU/µg throughout the entire process. In case we fail, there is no charge to you
Endotoxin removal is one of the most difficult tasks in downstream processes during protein purification. The removal of endotoxins becomes more challenging when associated with labile biomolecules, such as proteins. The form below lists number of approaches typically utilized to reduce endotoxin contamination of protein preparations. The success of these techniques in separating endotoxin from proteins is strongly dependent on the properties of the target protein.
|Ion exchange chromatography||It has rapid separation, wide selection of AEC media, sodium hydroxide (NaOH) sanitisation and does not require any solvents|
|Hydrophobic chromatography||Interacts with non-polar protein surfaces through van der Waals forces for endotoxin removal|
|Gel filtration chromatography and ultrafiltration||
|Triton X-114 phase separation||
The commonly used FDA-approved techniques for endotoxin detection are the rabbit pyrogen test and Limulus Amoebocyte Lysate (LAL) assay.
|Rabbit pyrogen test||Measuring the rise in temperature of rabbits after intravenous injection of a test solution||
|Limulus Amoebocyte Lysate (LAL) assay||LAL gel-clot assay||Using the characteristics of limulus reagent can react with bacterial endotoxin, to detect the content of bacterial endotoxin||
|Turbidimetric LAL assay||
|Chromogenic LAL assay||
One-stop endotoxin removal service includes gene synthesis, protein expression and purification, and endotoxin detection and removal. Endotoxin control and removal starts from protein preparation experiment to ensure the low level of endotoxin. All the vessels, the instruments, water, reagents, liquid move straws used in the experiments need to do endotoxin removal. At the same time, the experiment is done in clean room.
General endotoxin removal service includes removing endotoxin with your target protein and quantitative and/or qualitative detection of endotoxin before and after endotoxin removal.
Generally, we guarantee to effectively reduce the endotoxin level to 1 EU/µg or less throughout controlling the endotoxin level from the protein preparation experiment.
1. Results of endotoxin removal throughout the entire process controlling
|Results (solidification or not)||Solidification||No solidification||No solidification||No solidification||No solidification|
2. Results of endotoxin removal after protein preparation
|Results (solidification or not)||Solidification||Solidification||Solidification||No solidification||No solidification|
Analysis of experiment results: by controlling endotoxin throughout entire process, bacterial endotoxin level in the protein was much lower than that simply removing endotoxin after protein preparation.