BiologicsCorp owns a self contained and complete optimization platform for supernatant protein expression which contains 9 host bacteria, 6 molecular chaperones, 9 soluble tags, dozens of vectors, as well as multiple temperatures and induction strategies. Combining the techniques, we have tested many soluble proteins that are normally expressed in inclusion bodies. More than 90% of them have resulted in at least one optimal condition under which high level of target protein expression is achieved in soluble fraction.
|Content||A complete set optimization for your protein of interest|
|Deliverable||Soluble protein (Option)
|Timeline||Customized according to your project|
|Additional services||Basic guaranteed package, Fermentation, Tag-free,
Endotoxin control, High-throughput protein production
|Codon optimization||produce pauses to slow down translation rate and balance the essential factors of soluble protein expression||Our in-house MaximaTM codon optimization program is developed to solve the problems for soluble protein expression.|
|Strains||Choose the best fit strain based on your protein of interest to improve soluble expression level of native folding protein||
|Vectors||Choose the best fit vector based on your protein of interest to improve soluble expression level of native folding protein||
|Expression temperature||Slow down the rate of protein synthesis and folding kinetics, decreasing the hydrophobic interactions that are involved in soluble protein self-aggregation||Cold-inducible promoters are used to maximize protein expression under low temperature.|
|Inducer concentration||Reduction in cellular protein concentration||High inducer concentration is potentially toxic to E.coli and accelerate formation of inclusion bodies|
|Growth medium||Improve growth medium nutrient||
|Fusion protein||Work as solubility enhancing partners||More than 70% of the proteins tested showed high expression of soluble protein with at least two of nine fusion partners
Available fusion tags: GST, MBP, NusA, IF2, barnase, Pfg27-17, intein, Trx and CaBP.
|Co-expression||Assist initial folding in soluble protein expression||Molecular chaperones promote the proper isomerization and cellular targeting by transiently interacting with folding intermediates.
Foldases accelerate rate-limiting steps along the folding pathway.
After optimization, the soluble protein was expressed in supernatant.
After optimization, the soluble protein expression level in supernatant was significantly improved.