Production of Recombinant Proteins – Challenges and Solutions (PDF)
Abstract: Efficient strategies for the production of recombinant proteins are gaining increasing importance,as more applications that require high amounts of high-quality proteins reach the market. Higher production efficiencies and, consequently, lower costs of the final product are needed for obtaining a commercially viable process. In this chapter, common problems in recombinant protein production are reviewed and strategies for their solution are discussed. Such strategies include molecular biology techniques, as well as manipulation of the culture environment.
Recombinant protein expression and purification: A comprehensive review of affinity tags and microbial applications
Abstract: Protein fusion tags are indispensible tools used to improve recombinant protein expression yields, enable protein purification, and accelerate the characterization of protein structure and function. Solubility-enhancing tags, genetically engineered epitopes, and recombinant endoproteases have resulted in a versatile array of combinatorial elements that facilitate protein detection and purification in microbial hosts. In this comprehensive review, we evaluate the most frequently used solubility-enhancing and affinity tags. Furthermore, we provide summaries of well-characterized purification strategies that have been used to increase product yields and have widespread application in many areas of biotechnology including drug discovery, therapeutics, and pharmacology.
So you Need a Protein – A Guide to the Production of Recombinant Proteins
Abstract: The field of biotechnology owes a great deal to the ability to produce recombinant proteins, which can be made
in far greater abundance than many native proteins, and are more easily quality controlled. There is a great need for individual
proteins to be produced for research purposes. This review is aimed at researchers who are not experienced at protein
expression, but find that they have a need to produce a recombinant protein. We detail the major expression systems
that will be commonly used in the laboratory situation- bacterial, yeast and insect cell culture. The application of each,
and the relative advantages/disadvantages are discussed.