TNF-alpha | Tumor Necrosis Factor-alpha, human

Tumour Necrosis Factor alpha (TNF alpha), is an inflammatory cytokine produced by macrophages/monocytes during acute inflammation and is responsible for a diverse range of signalling events within cells, leading to necrosis or apoptosis. The protein is also important for resistance to viral infection and cancers. TNF-α is also involved in lipid metabolism, and coagulation. TNF-α exerts many of its effects by binding, as a trimer, to either a 55 kDa cell membrane receptor termed TNFR-1 or a 75 kDa cell membrane receptor termed TNFR-2. Both these receptors belong to the so-called TNF receptor superfamily.
Full Name Tumor Necrosis Factor-Alpha Human Recombinant
Synonyms TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2.
Source E. coli
Amino Acid Sequence MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA E AKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL.
Molecular Weight Tumor Necrosis Factor-α Human Recombinant was produced in E. coli as a single, non-glycosylated, polypeptide chain containing 158 amino acids (157 amino acids of the mature human TNF-alpha and an N-terminal methionine) and shown to have a molecular mass of 17.5 kDa.
Purity >95% as determined by SDS-PAGE and HPLC
Endotoxin Endotoxin level was found to be ≤ 0.1 ng/µg(1EU/µg) by a LAL gel clot method.
Formulation Lyophilized from a 1xPBS filtered with a membrane (0.2 µm).
Reconstitution To reconstitute the protein, add sterile water to the lyophilized protein and make a preparation with a concentration ≥0.1mg/ml. The preparation can be diluted into other appropriate buffers if necessary. Cautions should be taken to avoid bacterial growth, resulting in potential contamination with endotoxin.
Stability Once lyophilized, the protein is stable at room temperature (≈3 weeks) and at -20°C (≥ 2 years from the date of receipt).Upon reconstitution, it can be stored at 4°C (≤ 7 days) or at -20°C (≤ 6 months). For long term storage, please store at -80°C and the addition of a carrier protein (0.1% HSA or BSA) is recommended.Avoid frequent freeze-thaw cycles.
Biological Activity The Specific Activity is >5.0x107 IU/mg as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D.
Usage FOR LABORATORY RESEARCH USE ONLY. Not intended for diagnostic or therapeutic purposes.

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Product Code: CY232
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